甘氨双唑钠对斑马鱼胚胎的作用  

Effect of sodium glycididazole on Zebrafish embryos

作　　者：李雨[1] 周诗妍[2] 贺兴东 

机构地区：[1]第二军医大学海军医学系放射医学教研室,上海200433 [2]中国科学院上海生命科学研究院国家斑马鱼模式动物中心,上海200031 [3]苏州深久医药生物技术有限公司,苏州215125

出　　处：《辐射研究与辐射工艺学报》2011年第4期249-253,共5页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金面上项目"切尔诺贝利核事故清理工作人员受照远后基因表达变化"(30270421)资助

摘　　要：观察乏氧型辐射增敏剂甘氨双唑钠(Sodium glycididazole,CMNa)对斑马鱼受精卵胚胎发育过程的作用。选取野生型斑马鱼受精卵,置入不同浓度的甘氨双唑钠溶液,接受不同剂量60Coγ射线照射,观察不同时间胚胎发育形态变化及71 h斑马鱼存活和形态,记录不同形态胚胎和成鱼发生率,重复实验并进行统计学处理。γ射线不同剂量(0、5、10和15 Gy)照射而未经CMNa作用斑马鱼受精卵71 h正常形态鱼孵出率分别为76%、52%、14%和0%;0.30 mmol/L CMNa水溶液单独作用对孵出率无明显影响,而5 Gy和10 Gy照射后置0.3 mmol/L CMNa水溶液中正常形态鱼孵出率则分别为40%和0%。该辐射剂量情况下0.3 mmol/L甘氨双唑钠辐射损伤效果明显高于对照组(p<0.01)。斑马鱼受精卵可能是一种有意义辐射增敏研究平台,本实验提示一个值得关注的新线索,尚需对这些变化发生背景、形态特征以及形成机制进行进一步详细分析。The effect of sodium glycididazole （CMNa） on Zebrafish embryos was investigated. The wild-type Zebrafish eggs were placed in different concentrations of sodium glycididazole and received different doses of 60Co γ-rays irradiation. The morphological changes of embryonic development at different time and the survival rate were recorded at the 71 h. The repeat experiments and statistical analysis were further made. It was observed that the hatching rate of the normal form of the fish was 76%, 52%, 14% and 0% after exposure to γ-rays for 71 h with 0, 5, 10 and 15 Gy, respectively. And relative hatching rate was 40% and 0% after exposure to y-rays with 5 and 10 Gy respectively when the samples treated with aqueous solution of 0.30 mmol/L CMNa. The radio-sensitive effects with treatment of 0.3 mmol/L CMNa was significantly higher than that in control （p〈0.01）. Zebrafish eggs may be suitable for the study of radio-sensitive effect as a special model. And it should take more attention as CMNa has a significant radio-sensitive effect on Zebrafish embryos.

关 键 词：甘氨双唑钠(CMNa) 斑马鱼胚胎 辐射增敏 

分 类 号：Q691.5[生物学—生物物理学] Q331