猪TLR4基因可变剪接体的鉴定  

Identification of Alternative Splicing Isoforms of Pig TLR4 mRNA

作　　者：杨秀芹[1] 翟春媛[1] 李海涛[1] 刘娣 

机构地区：[1]东北农业大学动物科技学院,哈尔滨150030 [2]黑龙江省农业科学院,哈尔滨150086

出　　处：《畜牧兽医学报》2011年第8期1051-1056,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：黑龙江省自然科学基金(200927);哈尔滨市科技创新人才研究专项基金(2008RFQXN018);中国博士后科学基金(200902426)

摘　　要：为合理、有效地利用民猪资源进行抗病育种,本研究利用重叠延伸RT-PCR结合巢式PCR法扩增民猪TLR4基因,寻找可变剪接体;利用竞争性RT-PCR方法研究各可变剪接体的组织表达情况。获得了猪TLR4基因3个可变剪接体(其中2个是未见报道的),并且鉴定出一类特殊的剪接模式——外显子内部的部分片段被单独剪除,该类型在动物中尚属首次发现;组织表达分析表明3个可变剪接体普遍表达。TLR4基因存在着复杂的剪接机制,也暗示着其功能的复杂性。In order to make use of hereditary resource of Min pig effectively,this study was designed to clone TLR4 gene of Min pig and to identify its alternative splicing isoforms with overlap extension RT-PCR combined with nested PCR methods,and to characterize its tissue expression pattern with competitive RT-PCR method.Three splicing isoforms of TLR4 gene were identified in Min pig.At the same time,a special splicing mechanism,excision of inside segment of a whole exon,was found.Competitive RT-PCR analysis showed that three isoforms ubiquitously expressed in all tissues detected.The results indicate the complexity of structure and function of pig TLR4 gene.

关 键 词：民猪 TLR4基因 可变剪接 组织表达 

分 类 号：S828[农业科学—畜牧学] Q754[农业科学—畜牧兽医]