一种用于非洲猪瘟病毒检测的PCR方法  

One PCR Method Used for Detection of African Swine Fever Virus

作　　者：杨吉飞[1] 关贵全[1] 刘志杰[1] 汪月凤[1] 李有全[1] 马米玲[1] 刘爱红[1] 任巧云[1] 陈泽[1] 苟惠天[1] 杜鹏飞[1] 罗建勋[1] 殷宏[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室甘肃省动物寄生虫病重点实验室农业部草食动物疫病重点实验室,兰州730046

出　　处：《畜牧兽医学报》2011年第8期1201-1206,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："948"项目(2010-S04);ASFRISK(211691);"973"项目(2010CB530206);国家自然科学基金项目(30800820;30972182;31072130;31001061);甘肃省重点项目(1002NKDA035;0801NKDA033);农业部肉牛牦牛产业体系项目(CARS-38);科技部合作专项;欧盟EPIZONE(FOOD-CT-2006-016236);ARBOZOONET(211757);PIROVAC(KBBE-3-245145)

摘　　要：基于非洲猪瘟病毒(ASFV)VP72基因设计引物,建立一种检测非洲猪瘟病毒的PCR方法。应用本研究所建立的方法与OIE参考的方法进行比较,并对参考实验室提供的非洲猪瘟病毒17个分离株的基因组以及本实验室收集的野外样品进行检测。结果显示:本研究设计的引物具有良好的特异性,与猪的其他病原没有交叉反应;其敏感性与OIE参考的方法相当;所建立的PCR方法能够成功扩增非洲猪瘟病毒17个分离株的基因组,野外样品检测均为阴性。根据上述研究结果,本研究所建立的方法具有很好的应用性,能够用于非洲猪瘟疫病的诊断以及防控。A polymerase chain reaction（PCR） was developed for detection of African swine fever virus（ASFV） based on the VP72 gene.This method was compared with the PCR method which was recommended for diagnosis of African swine fever by OIE.Seventeen genomic DNAs of ASFV isolates from ASF reference laboratory and some field samples collected from China were detected by PCR,respectively.The results revealed that it had a good specificity,no cross-reaction was observed with other pig pathogens.The sensitivity was equivalent to the PCR which was recommended by OIE.Seventeen genomic DNAs of ASFV isolates could be detected successfully,but all of the field samples were negative.This method has a good applicability and could be used in diagnosis,prevention and control of African swine fever.

关 键 词：聚合酶链式反应 非洲猪瘟 诊断 

分 类 号：S852.659.1[农业科学—基础兽医学]