DKP对3株病原菌生物膜的抑制作用研究  

Study on the Inhibition Effect of DKP on the Biofilms Formed by Three Pathogens

作　　者：王建华[1,2] 权春善[3] 赵朋超[1,2] 范圣第[3] 

机构地区：[1]中国科学院大连化学物理研究所,大连116023 [2]中国科学院研究生院,北京100049 [3]大连民族学院生命科学学院,大连116600

出　　处：《中国生物工程杂志》2011年第8期61-65,共5页China Biotechnology

基　　金：教育部重点项目基金资助项目(209153)

摘　　要：生物膜的存在使一些由病原菌引发的疾病变得更加难以治疗。经研究发现一种环二肽物质DKP——cyclo(Pro-Phe)能够抑制这3株病原菌(Staphylococcus aureus,Pseudomonas aeruginosa,Candida albicans)生物膜的形成。通过对不同浓度DKP作用下所形成的生物膜进行结晶紫定量、菌落计数分析和结构显微分析表明:在DKP的浓度达到10 mg/ml时,S.aureus和P.aeruginosa的生物膜几乎消失;在DKP的浓度达到12 mg/ml时,C.albicans的生物膜被显著抑制。这一发现为寻找新型的生物膜抑制剂治愈顽固疾病带来了新的希望。The biofilms make it more difficult to treat the diseases caused by pathogens with antibiotics.A kind of diketopiperazine（DKP）——cyclo（Pro-Phe）were found can inhibit the biofilms formation of Staphylococcus aureus,Pseudomonas aeruginosa and Candida albicans.The results of crystal violet staining,CFU（colony forming unit）analysis,and the structure analysis by optical microscope and atomic force microscope indicated that the biofilms of S.aureus and P.aeruginosa were almost disappeared with 10 mg/ml DKP,and the biofilms of C.albicans was significantly inhibited by 12 mg/ml DKP.This brings hope to the research work of novel biofilm inhibitors and cure of the biofilms-associated infections.

关 键 词：生物膜 病原菌 DKP 生物膜抑制剂 

分 类 号：Q734[生物学—分子生物学]