中药复方益糖康对糖尿病大鼠白色脂肪中脂联素、脂联素受体1基因表达的影响  

Effect of Chinese medicine Yi Tang Kang on white fat adiponectin and adiponectin receptor 1 gene expression in diabetic rat

作　　者：张冰冰[1] 石岩[2] 

机构地区：[1]辽宁中医药大学基础医学院中医基础教研室,沈阳110847 [2]辽宁中医药大学,沈阳110847

出　　处：《中华中医药杂志》2011年第8期1825-1827,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：辽宁省科学技术基金课题(No.20082063)~~

摘　　要：目的:观察中药复方"益糖康"对糖尿病大鼠白色脂肪脂联素(Adiponectin)和脂联素受体1(adiponectin receptor1,AdipoR1)的基因表达的影响。方法:大鼠高脂饲料饲养4周后,一次性腹腔注射链尿佐菌素(STZ)造模,将造模成功的大鼠用随机数字表法将其随机分为模型组和益糖康治疗组。空白对照组和模型组以蒸馏水灌胃,治疗组的大鼠用益糖康煎剂灌胃,4周后3组大鼠睾周白色脂肪组织放入液氮中保存,用RT-PCR法检测脂肪中脂联素和脂联素受体1mRNA表达。结果:治疗组的脂肪组织脂联素mRNA含量与空白对照组比较,没有显著差异,模型组较治疗组明显降低(P<0.01)。脂联素受体1mRNA表达与空白对照组的比较没有显著差异,模型组较空白对照组明显降低(P<0.01)。结论:推测中药复方"益糖康"具有上调白色脂肪脂联素和脂联素受体1基因表达的作用,这可能是益糖康治疗糖尿病早期大血管病变的机制之一。Objective：To observe the effect of ＂Yi Tang Kang＂ in diabetic rat white adipose adiponectin and adiponectin R1 of gene expression;Methods：Wistar rats of clean grade 40 with high fat diet for 4 weeks after intraperitoneal injection of streptozotocin（STZ） modeling,the model was successful in rats using random number table to randomly divided into model group and treatment group health benefits of sugar.Control group and model group to distilled water,rats treated with decoction health benefits of sugar,4 weeks after three weeks of white adipose tissue of rat testes into liquid nitrogen for preservation,using RT-PCR assay fat adiponectin and adiponectin receptor expression 1mRNA.Results：The treatment group adipose tissue adiponectin mRNA levels compared with the normal group,no significant difference between the two groups,model group than the treatment group was significantly lower（P0.01）.Expression of adiponectin receptors 1mRNA in comparison with the normal group,the treatment group and control group were not significantly different,model group was significantly lower than normal（P0.01）.Conclusion：The suggested Chinese Medicine ＂Yi Tang Kang＂ has raised the white adipose adiponectin and adiponectin receptor 1 gene expression,which may be beneficial in early treatment of diabetes sugar health mechanism of macrovascular disease.

关 键 词：益糖康 脂联素 脂联素受体1 大血管病变 

分 类 号：R285.5[医药卫生—中药学]