一种新型丝素支架材料的体内降解试验  

In Vivo Degradation Test of a Novel Silk Fibroin Scaffold

作　　者：杨磊[1] 陈宇[2] 朱良均[1] 杨明英[1] 张海萍[1] 邓连霞[1] 闵思佳[1] 

机构地区：[1]浙江大学动物科学学院,杭州310029 [2]浙江省中医药研究院,杭州310007

出　　处：《蚕业科学》2011年第4期713-718,共6页ACTA SERICOLOGICA SINICA

基　　金：国家自然科学基金项目(No.30870628)

摘　　要：将制备的冷冻致孔新型丝素支架材料植入兔耳皮下进行体内降解试验,评价丝素支架材料的降解规律以及生物相容性。对植入丝素支架材料的兔耳进行肉眼外观观察、组织切片观察和扫描电子显微镜观察。肉眼外观观察发现植入丝素支架材料部位的皮肤无明显红肿,经过28周左右皮下材料植入部位的突起逐渐消失;组织切片观察发现该丝素支架材料引起的组织反应较弱;扫描电子显微镜在28周时只观察到将近消失的丝素支架材料种植腔,表明材料已基本降解。相比之下28周时丝素膜仍完好,无明显降解。研究结果表明,冷冻致孔新型丝素支架材料有望开发为一种生物相容性优良的可降解吸收性组织工程支架材料。In order to evaluate the degradation pattern and biological compatibility of silk fibroin scaffolds,a novel frozen and pore-inducing silk fibroin scaffold was prepared and implanted into the subcutaneous tissue of rabbit ear to carry out the in vivo degradation test.Consequently,the implanted silk fibroin scaffold was observed via naked eyes,histological slicing and scanning electron microscopy.Naked eye observation showed that no significant red swelling was observed under the skin implanted with silk fibroin scaffold and the salient with subcutaneous implant gradually disappeared in about 28 weeks.Histological slice observation showed that tissue response induced by the silk fibroin scaffold was limited.Scanning electron microscopy only showed the implant＇s cavity which was close to disappear at 28 weeks,indicating that almost all the scaffold had been degraded.On the contrary,the silk fibroin membranes were still complete and no obvious degradation was observed at 28 weeks.These results suggest that the novel frozen and pore-inducing silk fibroin scaffold is a promising material for preparing histological engineering scaffolds with excellent biocompatibility and biodegradability.

关 键 词：丝素支架 降解性 生物相容性 肉眼观察 组织切片 扫描电子显微镜 

分 类 号：R318.08[医药卫生—生物医学工程] S881.3[医药卫生—基础医学]