复方藤梨根制剂对胃癌细胞乙酰肝素酶、基质金属蛋白酶-9mRNA表达的影响  

Effect of Compound TengLiGen Recipe on Gene Expression of Heparitinase and Matrix Metalloproteinase-9 in Gastric Cancer Cell

作　　者：徐玲[1] 郭勇[2] 秦建领[3] 方勇[4] 潘宏铭[4] 

机构地区：[1]浙江省中西医结合医院肿瘤内科,浙江杭州310003 [2]浙江中医药大学附属第一医院肿瘤内科,浙江杭州310006 [3]郑州市第三人民医院,河南郑州450000 [4]浙江大学医学院附属邵逸夫医院肿瘤内科,浙江杭州310016

出　　处：《中华中医药学刊》2011年第8期1798-1800,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省卫生厅中医药青年基金研究计划资助项目(2005A12)

摘　　要：目的:探讨复方藤梨根制剂抑制胃癌转移机制。方法:建立裸鼠胃癌腹腔移植瘤模型24只,随机分成生理盐水组、复方藤梨根制剂低剂量、中剂量和高剂量组;18天后,给予复方藤梨根制剂灌胃21天。随后观察裸鼠生存天数和死亡后肿瘤转移浸润情况;用RT-PCR方法检测各组肿瘤组织中HPA、MMP-9 mRNA的表达。结果:对照组与低剂量治疗组转移部位的赋值评分显著高于中、高剂量组;对照组胃癌细胞的HPA mRNA表达高于中、高剂量组(P<0.05);对照组胃癌细胞的MMP-9 mRNA表达明显高于各给药组(P<0.05)。结论复方藤梨根制剂可能通过下调HPA、MMP-9基因的表达而抑制胃癌转移并延长生存时间。ObjectiveTo investagate the mechanism of Compound TengLiGen Recipe on metastasis of gastric cancer.Method24 models of the intraperitoneal xenografts of nude mouse were randomly devided into saline,low dose group,medium dose group and high dose group.After 18 days,the four groups were treated by Compound TengLiGen Recipe intragastric administration respectively for 21 days,Record the change of number of survival days,and metastasis/infiltrating status after death.Detect the mRNA expression of HPA MMP-9 in the tumor tissue after death.ResultAfter the treatment of 3 weeks,the score of metastasis/infiltrating of control group and low dose grope were higher than medium and high dose group;the mRNA expression of HPA of control group is higher than medium and high dose group（P0.05）;the mRNA expression of MMP-9 of control group is higher than each treated group（P0.05）.ConclusionCompound TengLiGen Recipe down regulation the gene expression of HPA and MMP-9 can be one of the reasons of its effects that anti-metastasis,prolong the survival time of gastric cancer patients.

关 键 词：复方藤梨根制剂 胃癌 乙酰肝素酶 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]