川芎嗪对咪达唑仑所致记忆障碍小鼠学习功能的影响  

The effects of tetramethylpyrazine on midazolam-induced dysmnesia in mice

作　　者：蒋月明[1] 郭根花[1] 王树桂[1] 万晶[1] 姚毅[1] 周兴[1] 宋潇[1] 彭飞飞[1] 刘金东[2] 马涛[3] 袁力勇[4] 

机构地区：[1]徐州医学院麻醉学院,江苏徐州221002 [2]徐州医学院附属医院麻醉科,江苏徐州221002 [3]徐州医学院麻醉药理学教研室,江苏徐州221002 [4]宁波市妇女儿童医院麻醉科,浙江宁波315012

出　　处：《中华中医药学刊》2011年第8期1823-1825,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：江苏省高等学校大学生实践创新训练计划项目(2010798);宁波市医学科技计划项目(2007014)

摘　　要：目的:观察川芎嗪注射液(tetramethylpyrazine,T)对咪达唑仑(midazolam,M)所致记忆障碍小鼠学习功能的影响。方法:将小鼠分为4组:生理盐水组(NS组),咪达唑仑组(M组),川芎嗪50 mg.kg-1+咪达唑仑组(T50+M组),川芎嗪100 mg.kg-1+咪达唑仑组(T100+M组)。用避暗实验(step-through test)观察各组24h、48h的潜伏期(latency)和错误次数(error times)。结果:24h,与NS组相比,M组、T50+M组、T100+M组潜伏期明显缩短(P<0.01),错误次数增加(P<0.01或P<0.05);与M组或T50+M组相比,T100+M组错误次数明显增加(P<0.01);48h,与NS组相比,M组潜伏期缩短(P<0.05),错误次数明显增多(P<0.01),T50+M组或T100+M组两项指标均无统计学差异(P>0.05);与M组相比,T50+M组错误次数减少(P<0.05)。结论:大剂量川芎嗪能增强咪达唑仑的遗忘作用,24h后却表现出记忆保护的趋势。Objective：To investigate the effects of tetramethylpyrazine on midazolam-induced dysmnesia in mice.Methods：The mice were divided into 4 groups（n=10）： normal saline group（NS group）,midazolam group（M group）,tetramethylpyrazine 50mg kg-1＋ midazolam group（T50＋M group） and tetramethylpyrazine 100mg kg-1＋ midazolam group（T100＋M group）.The latency and error times of each group were observed by the step-through test.Resuts： Compared with NS group at 24h,the lateny of M group,T50＋M group and T100＋M group decreased significantly（P 0.01）,the error times increased（P 0.01 or P 0.05）;compared with M group or T50＋M group,the error times of T100＋M group increased obviously（P 0.01）.Compared with NS group at 48h,the latency of M group shorted（P 0.05） and the error times increased obviously（P 0.01）,but there were no statistical differences in NS group,T50＋M group and T100＋M group（P0.05）;Compared with M group,the error times of T50＋M group decreased（P 0.05）.Conclusion： Combination high dose tetramethylpyrazine with midazolam can enhance midazolam-induced dysmnesia but 24h later can improve the learning deficit and dysmnesia.

关 键 词：川芎嗪 咪达唑仑 避暗实验 学习记忆 遗忘 

分 类 号：R285.5[医药卫生—中药学]