阳离子型抗菌剂聚六亚甲基双胍与核酸结合的协同作用  

Cooperativity in the Binding of the Cationic Biocide Polyhexamethylene Biguanide to Nucleic Acids

作　　者：Michael J. Allen Andrew P. Morby Graham F. White 江正武(译)[2,3] 赵春霞(译)[2,3] 刘白玲(校)[3] 

机构地区：[1]Cardiff School of Biosciences, Cardiff University, Cardiff CFIO 3US, UK [2]中国科学院研究生院,北京100039 [3]中国科学院成都有机化学研究所,四川成都610041

出　　处：《中国皮革》2011年第17期45-51,共7页China Leather

摘　　要：研究了广谱抗菌剂聚六亚甲基双胍(PHMB)与各类核酸的相互作用。通过滴定反应研究了PHMB与具有100个碱基(对)的单(双)链DNA、混合长度的标准DNA、tRNA相互作用形成的复合物沉淀。试验发现:PHMB与核酸之间是核苷酸/双胍基团以单元比相结合的。它们之间的这种结合具有很强的协同性,其表观Hill系数为10.3～14.6。用核酸滴定PHMB的荧光衍生物时,四类核酸的作用都显示了很强的荧光偏振强度,表明了核酸与PHMB之间的结合作用。PHMB与各类核酸之间强烈而广泛的结合作用,对这类抗菌剂的作用机理有着重要意义。The interaction between the broad - spectrum antimicrobial agent, polyhexamethylene biguanide （ PHMB）, and various nucleic acids was investigated. Titration of either single - or double - stranded 100 - bp DNA, or mixed - molecular weight marker DNA, or tRNA with PHMB caused precipitation of a complex between nucleic acid and PHMB in which the nucleotide/ biguanide ratio was always close to unity. Binding of PHMB was highly cooperative, with apparent Hill coefficients I0.3 - 14.6. When a fluorescent derivative of PHMB was titrated with increasing amounts of nucleic acid, all four forms of nucleic acid caused strong polarisation of fluorescence, demonstrating the association with PHMB. The intensity and broad - spectrum binding of PHMB to all forms of nucleic acid has significant implications for the mechanism of action of this biocide.

关 键 词：抗菌剂 聚六亚甲基双胍 阳离子表面活性剂 DNA结合 协同性 

分 类 号：TS529[轻工技术与工程—皮革化学与工程]