生物细胞亚类识别多维光学传感光路优化设计  

Optimal design of a multidimensional optical sensing system in biological cell subclass identification

作　　者：王亚伟[1] 王先凯[1] 尚学府[1] 卜敏[1] 田相龙[2] 姜守望[2] 

机构地区：[1]江苏大学理学院,江苏镇江212013 [2]江苏大学机械学院,江苏镇江212013

出　　处：《激光杂志》2011年第4期41-44,共4页Laser Journal

基　　金：江苏省高校自然科学重大项目(09KJA14001);江苏省高校光学制造重点实验室开放课题(kjs0905)

摘　　要：针对生物细胞原位检测和提高分类精度这一研究热点,以白细胞为主要研究对象,利用细胞光散射识别方法,根据细胞各方向散射光的特点,设计了一种多维光学传感系统,即是通过该光学系统可以检测细胞前向大小角,侧向偏振动,以及后向散射光。其中对各向光路系统构成和各个元件参数进行了优化设计,使系统的分辨率在80lp/mm时,MTF值不低于0.18,满足检测要求。同时后向散射光的接收光功率提高为原来的2倍,有效滤除杂散光,保证高信噪比信号的提取。优化设计后的多维光学传感系统可应用于白细胞的精确识别科学技术领域。In order to improve classification precision and in situ detection of biological cells,Leukocyte subclasses are analyzed by light scattering identification method.A multidimensional optical sensing system is designed based on the characteristics of their scattering light.It can detect forward small and large angle scatter,polarized side scatter and back scatter.After optimal design of optic system structure and each element parameters,when the resolution is 80lp/mm,the MTF value is not less than 0.18.The optical efficiency of back receiver system increases 1 time.The stray light is filtered out effectively and scattering signals with a good signal-to-noise ratio can be extracted.The optical system optimized can be used in scientific research of Leukocyte identification.

关 键 词：光散射 血细胞 分类识别 多维传感 光学系统 

分 类 号：TN202[电子电信—物理电子学] TH776[机械工程—仪器科学与技术]