NOS抑制剂对甲醛炎性痛诱导的大鼠痛反应及脊髓神经元凋亡的影响  

Effects of nitric oxide on spontaneous pain reaction and neuronal apoptosis in the spinal cord of rats induced by formalin inflammatory pain

作　　者：褚赛纯[1] 胡玉燕[1] 李清君[1] 李慧娜[1] 李文斌[1] 

机构地区：[1]河北医科大学基础医学院病理生理学教研室,石家庄050017

出　　处：《中国应用生理学杂志》2011年第3期372-375,共4页Chinese Journal of Applied Physiology

基　　金：河北省科技攻关课题(06276102D-40)

摘　　要：目的:观察甲醛炎性痛是否可诱导脊髓神经元凋亡以及一氧化氮(NO)对甲醛炎性痛诱导的大鼠痛反应以及脊髓神经元凋亡的影响。方法:采用行为学方法观察大鼠自发痛反应,流式细胞术检测脊髓神经元凋亡率。结果:与正常大鼠比较,甲醛炎性痛可诱导大鼠脊髓神经元凋亡率明显增加,于注射甲醛后3 d时最为明显。预先鞘内注射NOS抑制剂L-NAME可剂量依赖性抑制足底注射甲醛诱导的大鼠第一相和第二相痛反应,并可剂量依赖性抑制足底注射甲醛诱导的脊髓神经元凋亡过程;正常大鼠鞘内注射L-Arg也可诱发出伤害性反应和脊髓神经元凋亡。结论:甲醛炎性痛可诱导脊髓神经元发生凋亡,于注射甲醛后3 d神经元凋亡最为明显;炎性痛诱导的NO产生增多促进了脊髓伤害性信息传递过程,并在炎性痛诱导的脊髓神经元凋亡过程中发挥促进作用。Objective： To observe whether formalin inflammatory pain can induce neuron apoptosis in rats spinal cord or not and the effects of nitric oxide on the spontaneous pain reaction and neuron apoptosis in the spinal cord of rats with formalin inflammatory pain. Methods： Formalin-induced paw licking time was used to reflect the degree of spontaneous pain of rats, and the flow cytometry was used to detecte neuron apoptosis rate of spinal cord. Results： Compared with control group, the apoptosis ratio of spinal neuron was increased in the rats with formalin inflammatory pain, and peaked at 3d after formalin injection. Pre-intrathecal injection of NOS inhibitor L-NAME inhibited the uociceptive behavioural response in double phases induced by forrnahn injection and cut down the neuron apoptosis ratio of spinal cord of rats with formalin inflammatory pain. Nociceptive behavioural response and incraesed neuron apoptosis in the spinal cord were induced by intrathecal injection of L- Arg in normal rats. Conclusion： The results indicated that formalin inflammatory pain could induce the apoptosis of spinal neurons. The neurons apoptosis was the most significant on the third day after formalin injection. The increased pruduction of NO in spinal cord could promote the transmit of nociceptive information and participate the induction of neuronal apoptosis during the formalin inflammatory pain.

关 键 词：甲醛炎性痛 脊髓 神经元凋亡 

分 类 号：R338.3[医药卫生—人体生理学]