不同基因型大豆GS_1基因的克隆与分析  

Cloning and analysis of GS_1 genes from different soybean varieties

作　　者：陈丽华 刘丽君[2] 刘页丽[2] 裴宇峰 祖伟[2] 

机构地区：[1]国家农业标准化监测与研究中心,哈尔滨150036 [2]东北农业大学农学院,哈尔滨150030 [3]黑龙江农垦科研育种中心,哈尔滨150090

出　　处：《东北农业大学学报》2011年第7期101-106,共6页Journal of Northeast Agricultural University

基　　金：国家"十一五"科技支撑计划资助项目(2007BAD89B05);黑龙江省教育厅科研项目(11511029)

摘　　要：克隆了6个不同基因型大豆的GS1基因,序列比对分析结果表明,不同基因型大豆的GS1基因序列相似性较高。不同基因型大豆GS1基因序列间仍存在显著的差异,主要集中在非编码区。GS1基因分子进化树显示东农42、半野生大豆基因序列进化距离较近。不同基因型大豆GS1蛋白质序列具有很高的相似性,并且都具有两个保守的结构域、催化结构域(Gln-synt_N)和结合结构域(Gln-synt_C),说明它们都具有催化功能。GS1蛋白质序列的分子进化树显示东农46和东农42 GS1蛋白质序列进化距离较近,垦丰9和黑农35 GS1蛋白质序列进化距离较近。克隆了东农42 GS1基因的基因组序列,NetGene2软件、DNAMan软件的Dot metrix功能和NCBI在线spidey软件预测和分析结果表明,该基因具有12个外显子和11个内含子,外显子侧翼序列都符合GT、AG规则。GS1 genes from six different soybean varieties were cloned.The compare analysis of gene sequence showed that： sequence of GS1 genes from different soybean varieties were very identical.GS1 gene sequence from different soybean varieties still existed distinct difference which site mostly in non-coding region.Molecular evolutionary tree showed that evolution distance of GS1 genes sequence from DN42 and G.gracili were relatively near,they all had two conserved domains,catalyse domain（Gln-synt_N）and combine domain（Gln-synt_C）,and showed that they all had function of catalyse.Molecular evolution tree showed that evolution distance of GS1 protein sequence of DN42 and DN46 was near,KF9 and HN35 was near.GS1 genome sequence of DN42 was cloned.The forecast result through NetGene2 software,Dot metrix function of DNAMan software and NCBI online spidey software showed that this gene had 12 exons and 11 introns,the flank sequence of exon were all accord with GT,AG rule.

关 键 词：大豆 GS1基因 克隆 

分 类 号：S565.1[农业科学—作物学] Q785[生物学—分子生物学]