慢性间断缺氧对大鼠脑皮层NF-κB、ICAM-1和TNF-α表达的影响  

Effects of Chronic Intermittent Hypoxia on the Expression of NF-κB in Rat Cortex

作　　者：杨美丽[1] 吴振纲 邱建龙[3] 蔡若蔚[1] 

机构地区：[1]福建医科大学附属第二医院神经内科,泉州362000 [2]泉州医学高等专科学校,泉州362000 [3]福建医科大学附属第二医院病理科,泉州362000

出　　处：《福建医科大学学报》2011年第3期177-181,共5页Journal of Fujian Medical University

基　　金：泉州市科技项目(2007Z14);福建省教委资助项目(JA05269)

摘　　要：目的探索慢性间断性缺氧(CIH)对大鼠额顶部皮质-κB(NF-κB)、细胞间黏附分子-1(ICAM-1)和肿瘤坏死因子-α(TNF-α)表达的影响。方法制作CIH大鼠模型。将30只SD大鼠均分为CIH组(实验组)、空气模拟对照组及空白对照组。采用免疫组织化学方法检测3组大鼠额顶部皮质NF-κBI、CAM-1和TNF-α的表达,并行统计学处理。结果实验组大鼠额顶部皮质NF-κB表达高于空白对照组和空气模拟对照组(均为P<0.05),而空白对照组与空气模拟对照组之间比较,差别无统计学意义(P>0.05);实验组可见ICAM-1在额顶部皮质和基底节区微血管内皮细胞表面表达,另两组未见表达;实验组皮质的TNF-α亦显著高于另两组(P<0.05)。结论 CIH可通过上调大鼠脑皮层NF-κB的表达并促进其目的基因产物ICAM-1和TNF-α的表达介导脑损害。Objective To observe the effect of chronic intermittent hypoxia on NF-κB,ICAM-1,TNF-α expressions in cortex.Methods Thirty male SD rats were randomly divided into three groups： the experimental group,the air mimic group and the blank control group.Immunohistochemistry was used to examine the expression of nuclear transcription factor κB（NF-κB）,intercellular adhesion molecule-1（ICAM-1） and tumor necrosis factor-α（TNF-α）in the frontal and parietal cortex cells.Results The expression of NF-κB in cortex cells from the experimental group was significantly higher than those from the air mimic control group（P0.05） and the blank control group（P0.05）.The expression of NF-κB in cortex cells of the air mimic control group was not significantly different from those of the blank control group（P0.05）.The experimental group showed that ICAM-1 expression in the microvascular endothelial cells,mainly distributed in the frontoparietal cortex and basal ganglia.There was no positive expression of ICAM-1 in blood vessels in the blank control group and the air mimic control group.The expression of TNF-α in cortex cells from the experimental group was significantly increased compared with those from the air mimic control group and the blank control group（P0.05）.Conclusion CIH increases the expression of NF-κB and its target gene products ICAM-1 and TNF-α,and therefore affects the cerebral vascular system.

关 键 词：慢性间断缺氧 核转录因子 细胞间黏附分子 肿瘤坏死因子 

分 类 号：R56[医药卫生—呼吸系统]