豚鼠最大值试验法研究二氯乙酰氯皮肤致敏作用的初步报告  

Study on skin sensitization effect caused by dichloroacetyl chloride in guinea pigs using GPMT method

作　　者：黄永顺[1] 陈嘉斌[1] 谢植伟[1] 金佳纯[1] 睢罡[2] 符传东[2] 刘成武[3] 李娟[3] 

机构地区：[1]广东省职业病防治院,广东广州510300 [2]山西医科大学公共卫生学院,山西太原030001 [3]中山大学公共卫生学院,广东广州510080

出　　处：《中国工业医学杂志》2011年第4期253-255,共3页Chinese Journal of Industrial Medicine

基　　金：广东省医学科研基金立项课题(A2009057)

摘　　要：目的探讨二氯乙酰氯(DCAC)是否在三氯乙烯药疹样皮炎发病过程中起到半抗原的作用。方法按豚鼠最大值试验(GPMT)法,用DCAC对白色豚鼠进行皮内和涂皮结合法致敏试验,并设阳性对照组(二硝基氯苯)、阴性对照组(橄榄油和福氏完全佐剂),观察各组动物皮肤的红斑和水肿等情况,求出致敏率和平均反应值,并且检测ALT和AST以及计算各脏器系数。结果 DCAC实验组、阳性对照组和阴性对照组的致敏率分别为0%、100%和0%;平均反应值分别为0、6.0和0;各实验组豚鼠的ALT和AST以及脏器系数差异无统计学意义。结论 DCAC可能在三氯乙烯药疹样皮炎发病过程中没有起到半抗原的作用,并且对实验动物没有明显肝功能损害。Objective To explore whether the trichloroethylene induced medicamentosa-like dermatitis is initiated by DCAC as hapten or not.Methods A modified guinea pig maximization test（GPMT）was used,and the skin signs,such as edema and erythema were carefully examined in guinea pigs treated with DCAC,24-dinitrochlorobenzene（positive control）and olive oil（negative control group）,respectively.Meanwhile,the activities of ALT and AST in serum were also determined.Results The results showed that the sensitizing rates and mean response scores in DCAC treated group,positive control group and negative control group were 0%,100%,0%;and 0,6.0,0,respectively.The differences of ALT and AST as well as the organ coefficients among these groups were also not significant.Conclusion It was suggested that DCAC may not play a role of hapten during the onset of medicamentosa-like dermatitis caused by TCE.

关 键 词：豚鼠最大值试验 二氯乙酰氯 致敏 

分 类 号：R758.22[医药卫生—皮肤病学与性病学]