三种核酸共提取试剂盒对血液病毒提取效能的比较研究  

Comparison of Efficiency of Three Nucleic Acid Extraction Kits for Extracting Blood Virus

作　　者：王聪[1,2] 陈之遥 武海萍 周国华[1,2] 

机构地区：[1]中国药科大学生命科学技术学院,南京210009 [2]华东医学生物技术研究所,南京210002

出　　处：《临床误诊误治》2011年第8期6-9,共4页Clinical Misdiagnosis & Mistherapy

基　　金：江苏省科技支撑计划(BE2010605);江苏省自然科学基金(BK2010111)

摘　　要：目的比较不同核酸共提取试剂盒对血液病毒的提取效能差异。方法选择国内外广泛使用的3种血液病毒核酸共提取试剂盒,分别为OMEGA试剂盒、QIAGEN试剂盒和WATSON试剂盒,HBV阳性、HBV阴性、HCV阳性和HCV阴性血清样本均取自南京军区南京总医院。样本核酸提取按照各试剂盒说明书进行操作,定量方法采用实时荧光定量PCR。结果 OMEGA试剂盒对DNA的提取效率最高,对RNA的提取效率最低,耗时最短,成本居中;QIAGEN试剂盒DNA和RNA提取效率均较高,耗时居中,成本最高;国产WATSON试剂盒对DNA或RNA提取效率居中,耗时最长,成本最低。结论不同试剂盒对血液病毒的提取效能各不相同。对于已知病毒载量和病毒种类的样本,根据提取液DNA浓度和RNA浓度选择试剂盒;而对于未知病毒载量和病毒种类的样本,首选QIAGEN试剂盒,其次可选WATSON试剂盒。如只考虑成本,则首选WATSON试剂盒。Objective To compare the efficiency of different nucleic acid extraction kits for extracting blood virus. Methods 3 commonly used kits for extraction of blood viral nucleic acid were chosen from domestic： and international markets, including OMEGA kit, QIAGEN kit and WATSON kit, and serum samples with or without Hepatitis B virus （HBV） and serum samples with or without hepatitis C virus （HCV） were selected from General Hospital of Nanjing Military Area Com- mand. The nucleic acid extraction procedures were carried out according to the instructions of the 3 kits and the real-time quantitative PCR was performed for quantitative analysis. Results OMEGA kit had the best extraction efficiency of DNA but had the worst extraction efficiency for RNA extraction within a short time and at medium cost. OIAGEN kit had good efficiencyfor both DNA and RNA extraction with medium term, but at the highest cost; WATSON kit had medium efficiency rate for both DNA and RNA extraction with long term extraction and at the lowest cost. Conclusion There are different efficiency rates at the in extracting blood virus with different nucleic acid extraction kits. We should choose kits for the samples with clear virus capacity and category according to the concentrations of extracted DNA and RNA, but for the samples with unknown capacity and category of virus, QIAGEN kit should be the first priority and followed by WATSON kit. WATSON kit should be the first choice in terms of cost.

关 键 词：病毒核酸提取试剂盒 实时荧光定量聚合酶链反应 提取效率 

分 类 号：R457.11[医药卫生—治疗学]