ABO血型正反定型不符标准操作规程的建立与应用  

Establishment and Application of Standard Operating Procedure for Discrepancy of Forward and Reverse ABO Typing

作　　者：朱培元[1] 唐雯[1] 相晓[1] 栾建凤[1] 

机构地区：[1]南京军区南京总医院输血科,南京210002

出　　处：《临床误诊误治》2011年第8期9-11,共3页Clinical Misdiagnosis & Mistherapy

基　　金：全军"十一五"科技攻关项目(08Z020);南京军区医学科技创新重点资助项目(07Z033)

摘　　要：目的探讨ABO血型正反定型不符标准操作规程的建立。方法我院2010年10月～2011年4月检出14例正反定型不符样本，按ABO血型正反定型不符的标准操作规程进行处理，包括重新测试，核查标本、试剂，复习临床资料、分类和验证等。结果14例正反定型不符问题均得到解决。4例因技术原因所致，经过重新测试，核查样本和试剂，仔细判读结果，得到解决。余分别系多发性骨髓瘤等疾病（3例）、ABO亚型（2例）、同种异基因造血干细胞移植（2例）、先天性抗体缺乏（1例）、输异型血（1例）、cisAB型（1例）等导致。结论建立处理ABO血型正反定型不符的标准操作规程有助于规范疑难血型的鉴定过程，提高输血安全性。Objective To investigate the establishment of standard operating procedure（SOP） for discrepancy of forward and reverse ABO typing. Methods 14 blood samples of discrepant forward and reverse ABO typing were detected from October 2010 to April 2011 in our hospital. A SOP was used to treat the samples, including repeat testing, rechecking samples and reagents, and reviewing the clinical data, categorization and verification. Results All the ld samples were resolved. 4 samples caused by technical problems were treated by repeat testing, rechecking samples and reagents, and reading and reporting the results accurately. The other 10 samples were of disease states （e. g. multiple myeloma, n =3）, ABO subgroup （n = 2）, allogeneic hematopoietic stem cell transplantation （ n = 2）, congenital antibody deficiency （ n = 1 ）, non-specific blood transfusion （ n = 1 ） and cisAB （ n = 1 ） . Conclusion The SOP for forward arid reverse ABO typing can help to improve the ABO grouping process for the cases difficult to be identified and improve safety of transfusion.

关 键 词：ABO血型系统 血型鉴定和交叉配血 标准操作规程 

分 类 号：R446.6[医药卫生—诊断学]