温度对小新月菱形藻叶绿素荧光特性及生长的影响  

Effects of Temperature on Chlorophyll Fluorescence Parameters and Growth in Alga Nitzschia closterium f.minutissima

作　　者：梁英[1] 刁永芳[1] 陈书秀[1] 荣玲[1] 

机构地区：[1]中国海洋大学海水养殖教育部重点实验室,山东青岛266003

出　　处：《水产科学》2011年第8期435-440,共6页Fisheries Science

基　　金："十一五"国家科技支撑计划项目(2006BAD09A03)

摘　　要：以小新月菱形藻为试验材料,研究了其在一次性培养过程中,不同温度（5~30℃）对其叶绿素荧光参数[光系统Ⅱ的最大光能转化效率（Fv/Fm）、光系统Ⅱ的潜在活性（Fv/Fo）、光系统Ⅱ的实际光能转化效率（ΦPSⅡ）、相对光合电子传递效率（rETR）、光化学淬灭（qP）和非光化学淬灭（NPQ）]、叶绿素相对含量以及细胞密度的影响。单因子方差分析结果表明,在整个培养周期中,温度对小新月菱形藻各叶绿素荧光参数、细胞密度和叶绿素相对含量均有显著影响（P〈0.05）。多重比较结果表明,接种后1~2 d,20℃处理组的主要荧光参数（Fv/Fm、Fv/Fo、rETR、ΦPSⅡ）显著高于其他处理组。30℃的处理组的上述荧光参数从第1 d开始均显著低于其他处理组。20℃处理组的细胞密度和叶绿素相对含量均显著高于其他处理组。在本试验条件下,适宜小新月菱形藻生长的温度为10~25℃,最适温度为20℃。相关性分析结果表明,在整个培养周期中,小新月菱形藻的叶绿素相对含量和细胞密度之间存在显著的正相关。The chlorophyll fluorescence parameters（maximal photochemical efficiency of PSⅡ Fv/Fm,potential activity of PSⅡ Fv/Fo,actual photochemical efficiency of PSⅡΦPSⅡ,relative electron transport rate rETR,qP and NPQ）,the relative chlorophyll contents and cell density were studied in alga Nitzschia closterium f.minutissima under different temperatures（5~30 ℃）.One-factor analysis of variance revealed that the temperature had significant effects on the above parameters during the entire experiment（P0.05）.Multiple comparison showed that there were significantly higher Fv/Fm,Fv/Fo,ΦPSⅡand rETR under 20 ℃than those in other treatments from day 1 to day 2,while the above parameters were found significantly lower under 30 ℃ than those in other treatments from day 1 to the end of the experiment.The maximal relative chlorophyll content and cell density were observed in the alga in 20 ℃ treatment during the experiment,and the alga had the proper growth at 10~25 ℃,the best growth at temperature of 20 ℃.The correlation analysis indicated that there were significantly positive correlations between relative chlorophyll content and cell density.Furthermore,the utilization of chlorophyll fluorescence techniques in determination of suitable temperature range in microalgae,selection of high or low temperature resistance microalgal strains and the mechanism of temperature stress in algae were also discussed in this paper.

关 键 词：小新月菱形藻 温度 叶绿素荧光 生长 

分 类 号：S963.213[农业科学—水产养殖]