3-氨基苯甲酰胺影响糖尿病大鼠晶状体上皮细胞NF-κB表达的免疫组化研究  

The immunohistochemical study of the expression of NF-κB in the lens epithelial cells in diabetic rats by 3-AB

作　　者：覃冬[1] 康刚劲[1] 

机构地区：[1]泸州医学院附属医院眼科,四川泸州646000

出　　处：《重庆医学》2011年第24期2438-2440,共3页Chongqing medicine

基　　金：泸州市科技局资助[泸市科(2010)128号]

摘　　要：目的观察聚腺苷二磷酸核糖聚合酶(PARP)抑制剂3-氨基苯甲酰胺(3-AB)对早期糖尿病(DM)大鼠模型晶状体上皮细胞中核因子κB(NF-κB)表达的影响。方法采用一次性腹腔注射1%链脲佐菌素(STZ)50 mg/kg建立DM大鼠模型。成模后将大鼠随机分为正常对照组(N组)、糖尿病组(DM组)和干预组(DM+P组)。自建立模型后第3天起,DM+P组每日给予3-AB 30 mg/kg灌胃,N组和DM组每天给予等体积0.9%生理盐水灌胃。分别于给药后2、4、8周处死各组大鼠,取出晶状体,免疫组化SP法检测晶状体上皮细胞中NF-κB P65的表达情况。结果 DM 2、4、8周组大鼠晶状体上皮细胞NF-κB P65表达较相应时间点的N组高;DM+P 2、4、8周组大鼠晶状体上皮细胞NF-κB P65表达较相应时间点的N组高;DM+P 2、4、8周组与相应时间点DM组比较,晶状体上皮细胞NF-κB P65表达降低。结论 PARP抑制剂3-AB可以抑制早期糖尿病大鼠晶状体上皮细胞中NF-κB的表达。Objective To observe the effection of poly ADP ribose polymerase （poly ADP-ribose polymerase,PARP） inhibitor 3-aminobenzamide （3 AB） on the expression of nuclear factor Kappa B （nuclear factor-kappa B, NF-κB） in the early diabetic melli- tus（diabetes mellitus,DM） rats modal. Methods The rats were intervented by the intraperitional injection of 1% Stretozocin at a doze of 50 mg/kg. The rats were divided into N group,DM group,DM＋P group. Starting from the third day after modeling,DM＋ P group were given 3-AB 30 mg/kg per day orally. At the 2nd,4th,Sth week,the rats were sacrificed. Results The immunohistochemistry results showed that the expression of NF-κB p65 increased statistically in DM group than N group at the corresponding time points 2nd,4th,Sth week;The expression of NF-κB p65 in DM＋P group was significantly increased than N group at the 2nd, 4th,8th week. The expression of NF-κB p65 increased statistically in DM group at the 2nd,4th,8th week compared with the DM＋ P group. Conclusion PARP inhibitor 3-AB can inhibit the expression of NF-κB on the lens epithelial cells of the early DM rats.

关 键 词：NF-ΚB 糖尿病性白内障 聚腺苷二磷酸核糖聚合酶抑制剂核因子 晶状体上皮细胞 3-氨基苯甲酰胺 

分 类 号：R587.1[医药卫生—内分泌] R776.1[医药卫生—内科学]