小檗碱对CT26皮下移植瘤组织中肿瘤相关巨噬细胞的影响  

Berberine inhibits tumor-associated macrophages in subcutaneous tumor of mice

作　　者：李健[1] 郝钰[1] 张玉丽[2] 谢伟[2] 李军[2] 薛晓鸥[2] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]北京中医药大学附属东直门医院妇科,北京100700

出　　处：《中国组织化学与细胞化学杂志》2011年第3期203-206,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家科技支撑项目(2006BAI11B07-02);国家自然科学基金资助(30873268)

摘　　要：目的观察小檗碱的抑瘤作用及其对肿瘤组织内肿瘤相关巨噬细胞数量的影响。方法 BABL/c小鼠40只随机分2组,全部皮下移植结肠癌细胞(CT26细胞系),次日进行药物干预。治疗组小鼠腹腔注射小檗碱,100mmol/L,200μl/d,14d;对照组腹腔注射等量生理盐水。肿瘤细胞接种7d后连续动态测定肿瘤体积,接种15d处死全部动物,取肿瘤组织、免疫组织化学显色检测肿瘤组织中M2型巨噬细胞标志物CD206及CD68的表达。结果小鼠皮下移植CT26后,小檗碱治疗组小鼠皮下移植瘤生长缓慢。与对照组比较,肿瘤体积及瘤重均显著减少(P<0.05);皮下移植15d肿瘤组织中可见大量CD206及CD68阳性细胞;肿瘤组织中CD206及CD68阳性细胞数量显著减少(P<0.05 orP<0.01)。结论小檗碱可能通过抑制肿瘤相关巨噬细胞的形成而发挥抗肿瘤作用。Objective To observe the antitumor effect of berberine through inhibiting tumor-associated macrophages in subcutaneous tumor.Methods 40 BABL/c mice were divided into 2 groups： vehicle group and berberine treatment group.CT26 tumor cells were transplanted subcutaneously.Immunohistochemistry was used to examine tumor-associated macrophage phenotypes CD206 and CD68 in subcutaneous tumor tissue.Results Berberine inhibited tumor growth tendency remarkably（P0.05 vs.vehicle control）.The quantity of CD68＋/CD206＋ tumor-associated macrophages were reduced significantly by berberine treatment（P0.05 vs.vehicle control）.Conclusion The anti-tumor role of berberine is achieved by inhibiting the formation tumor-associated macrophages.

关 键 词：小檗碱 肿瘤相关巨噬细胞 CT26 

分 类 号：R285.5[医药卫生—中药学]