脊髓源神经干细胞诱导分化为胆碱能神经元过程中Aldoc和Stmn1表达的研究  

The expression of Aldoc and Stmn1 during the differentiation of spinal cord-derived neural stem cells into cholinergic neurons

作　　者：刘彩玉[1] 王春芳[1] 李鹏飞[1] 蔚洪恩[1] 龙志晶[1] 邓春圣[2] 

机构地区：[1]山西医科大学生物技术教研室,太原030001 [2]中国人民解放军第322医院,山西大同037006

出　　处：《中国组织化学与细胞化学杂志》2011年第3期252-256,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：山西省自然科学基金(2009011057-1);中国人民解放军第322医院自然科学基金(2011001)

摘　　要：目的探讨脊髓源神经干细胞在诱导分化为胆碱能神经元过程中Aldoc和Stmn1的表达变化情况。方法从孕17天Wistar胚胎大鼠取出脊髓组织,制成细胞悬液,采用含EGF和bFGF的无血清限定性培养基培养,然后进行诱导分化,观察脊髓源神经干细胞向胆碱能神经元分化的情况,应用荧光定量PCR方法分析Aldoc和Stmn1基因在脊髓源神经干细胞诱导分化为胆碱能神经元的过程中的表达变化情况。结果神经干细胞在诱导分化为胆碱能神经元后,经免疫荧光检测有ChAT阳性细胞表达;Aldoc基因的表达量在胆碱能神经元较神经干细胞低(P<0.05);Stmn1基因的表达量则在诱导分化后较神经干细胞升高(P<0.05)。结论 Aldoc对神经干细胞的干性维持有重要作用,Stmn1在胆碱能神经元的成熟过程中起作用。Objective To detect the expression changes of Aldoc and Stmn1 gene during the differentiation of neural stem cells derived from the embryonic spinal cord into cholinergic neurons in rats.Methods The neural stem cells were harvested from the spinal cord of 17-day-old fetal rats,cultured in a serum-free limited medium containing EGF and bFGF,and then induced by a conditioned medium.The real time quantitative PCR was used to detect the expression of Aldoc and Stmn1 mRNA during the differentiation.Results The cells were positive for ChAT by immunostaining analyses after the induction.The expression of Aldoc was decreased in cholinergic neurons（P0.05）;while the expression of Stmn1 was increased in cholinergic neurons（P0.05）.Conclusion Aldoc gene may play an important role in the maintenance of neural stem cells,whereas Stmn1 gene may play a role during the maturation process of cholinergic neurons.

关 键 词：神经干细胞 胆碱能神经元 Aldoc基因 Stmn1基因 基因表达 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]