慢性复合应激对成年海马FGF-2的表达及神经发生的影响  

Expression of FGF-2 in rat hippocampus after chronic multiple stress and its effect on neurogenesis

作　　者：肖娟娟[1] 王秋桂[1] 张敏海[2] 

机构地区：[1]咸宁学院基础医学院组织与胚胎学教研室,湖北437100 [2]华中科技大学同济医学院解剖学系组织与胚胎学教研室,武汉430030

出　　处：《中国组织化学与细胞化学杂志》2011年第3期281-285,共5页Chinese Journal of Histochemistry and Cytochemistry

基　　金：咸宁学院校级基金资助(KY0876)

摘　　要：目的通过观察慢性复合应激后大鼠海马FGF-2表达的变化,来探讨慢性复合应激对FGF-2表达的影响及其与海马神经发生的联系。方法成年雄性大鼠随机分为复合应激组和正常对照组。复合应激组动物每天交替无规律暴露于复合应激原中达6周。然后运用免疫组织化学方法、Western-blot和RT-PCR技术观察海马FGF-2表达的变化。结果慢性复合应激组动物海马FGF-2阳性细胞的表达量增多(P<0.05);海马FGF-2蛋白的表达明显增加(P<0.05);海马FGF-2 mRNA水平明显上调(P<0.05)。结论慢性复合应激可引起海马FGF-2阳性细胞表达量增加和FGF-2表达水平升高,提示应激后内源性FGF-2的表达增高可能是慢性复合应激促海马神经发生的因素之一。Objective To explore the relationship of FGF-2 with hippocampal neurogenesis by observing the effect of chronic multiple stress on the expression of FGF-2 in the hippocampus of rats.Methods Adult male Wistar rats were randomly divided into two groups： the multiple stressed group and the control group.Rats in the multiple stressed group were irregularly and alternatively exposed to the chronic multiple stress for 6 weeks.Then the expression of FGF-2 immunoreactive positive cells in the hippocampus of rats of two groups were compared by using immunohistochemisery,and the expressions of FGF-2 protein and mRNA in the hippocampus of rats were assayed by using Western-blot and RT-PCR respectively.Results（1） The expression of FGF-2 immunoreactive cells in the hippocampus of the multiple stressed rats was significantly higher than in the control group（P0.05）;（2） The protein and mRNA levels of FGF-2 in,the hippocampus of the multiple stressed rats were remarkably higher than those of the control group（P0.05）. Conclusion The enhanced expression of FGF-2 in the hippocampus may be induced by the chronic multiple stress,and it implies that endogenous FGF-2 may play a role in facilitating hippocampal neurogenesis in stressed rats.

关 键 词：慢性复合应激 海马 FGF-2 神经发生 大鼠 

分 类 号：R322.811[医药卫生—人体解剖和组织胚胎学]