PGN激活BV2内吞Aβ寡聚体后对PC12的影响  

Influences of peptidoglycan on PC12 cells after endocytosis of amyloid proteinβ oligomers in activated BV2 cells

作　　者：姜新[1] 谢明[1] 白丽娟[1] 陈晓虹[1] 马恩龙[2] 

机构地区：[1]辽宁省人民医院神经内科,沈阳110016 [2]沈阳药科大学药学院药理教研室,沈阳110016

出　　处：《首都医科大学学报》2011年第4期509-513,共5页Journal of Capital Medical University

基　　金：辽宁省科委基金资助2006科技研究(2006225003)~~

摘　　要：目的探讨前炎性介质肽聚糖(peptidoglycan,PGN)激活BV2细胞内吞β淀粉样蛋白(amyloid proteinβ,Aβ)1～42寡聚体后对PC12的影响。方法采用细胞株传代法培养PC12细胞及BV2细胞,分别替代神经元细胞和小胶质细胞;用转移筛网进行PC12、BV2细胞共育培养;制备Aβ1～42寡聚体;分别采用MTT方法检测PC12细胞抑制率、Western blotting方法检测各组PC12细胞tau(pS396)蛋白表达情况、流式细胞仪检测PC12细胞凋亡。结果 Aβ寡聚体、Aβ寡聚体作用BV2细胞后及PGN作用BV2细胞后均能抑制PC12细胞增殖、增加PC12细胞tau(pS396)表达量、增加PC12细胞凋亡率;而PGN激活BV2细胞内吞Aβ寡聚体后对PC12细胞增殖抑制、tau(pS396)表达量、PC12细胞凋亡率与前面三种情况比较明显增加。结论 Aβ寡聚体可引起PC12细胞的细胞抑制率,tau蛋白异常磷酸化水平,细胞凋亡的增多;PGN激活BV2细胞内吞Aβ后,加重Aβ寡聚体引起PC12细胞的细胞抑制率,tau蛋白异常磷酸化水平,细胞凋亡的增多。Objective To investigate how the pro-inflammatory medium of peptidoglycan（PGN） acts on PC12 after endocytosis of amyloid protein β（Aβ）oligomers in activated BV2 cells.Methods PC12 cells and BV2 cells,instead of neurons and microglia separately,were co-cultivated in transfer screen by cell passage method.Aβ1-42 oligomers were prepared according to Klein WL（2002）.The inhibition of PC12 cells was determined by MTT,and the expression of its tau protein was determined by Western blot,and the apoptosis of PC12 cells was determined by flow cytometry.Results Aβ oligomers,BV2 cells acted upon by Aβ oligomers,and BV2 cells affected by PGN all could restrain the proliferation of PC12 cells,increase the expression of its tau,and increase its apoptosis,while the effect of PGN after endocytosis of Aβ oligomers in activated BV2 cells were more remarkable.Conclusion Aβ oligomers can increase the inhibition of PC12 cells,abnormal phosphorylation of tau protein,and the apoptosis,and after endocytosis in activated BV2 cells by PGN,the effect of Aβ oligomers is more remarkable.

关 键 词：肽聚糖 小胶质细胞 β淀粉样蛋白1~42寡聚体 PC12细胞 细胞共育 

分 类 号：Q189[生物学—神经生物学]