马来酸氯苯那敏片剂的核磁共振定量测定方法研究  

Quantitation of Chlorphenamine Maleate in Tablet by NMR

作　　者：郭强胜[1] 石高旗[1] 宋巍[1] 许旭[1] 

机构地区：[1]上海应用技术学院化学与环境工程学院,上海200235

出　　处：《上海应用技术学院学报（自然科学版）》2011年第2期123-128,共6页Journal of Shanghai Institute of Technology: Natural Science

摘　　要：提出测定马来酸氯苯那敏片剂中马来酸氯苯那敏的1↑H-NMR方法。考察了实验条件的影响,选择重水为溶剂,反丁烯二酸为内标,延迟时间为20 s,脉冲宽度为3.0μs,采样次数为16次,内标浓度为4-5mg/mL。在内标与样品质量比为0.48-2.12范围内,峰面积比与质量比之间具有良好的线性关系,相关系数r〉0.999 0,用绝对定量法和标准曲线法测定了3种马来酸氯苯那敏片剂的含量,并与药典的紫外分光光度法作了比较。A simple and accurate proton-nuclear magnetic resonance（1↑H-NMR） method for quantitative determination of chlorphenamine maleate in tablet was presented.The method employed D2O as solvent and fumaric acid as internal standard.The delay time（D1） 1.0 s,pulse width（P1） 20 μs and number of transient（NS） 16 times were selected according to investigation.The relationship between NMR peak area ratio and mass ratio of fumaric acid to chlorphenamine maleate is linear with the correlation coefficient as above 0.9990 in the linear range of 0.48～2.12 mass ratio.Three kinds of real tablet were measured and the quantitative results obtained by means of 1↑H-NMR were found to be in agreement with absolute method of quantification.The ultraviolet spectrophotometry method used in pharmacopoeia was also compared.

关 键 词：核磁共振 定量 马来酸氯苯那敏 

分 类 号：R927.2[医药卫生—药学]