慢性前列腺炎患者血清中B7-H3分子的表达及其临床意义  

Expression of Soluble B7-H3 in Serum of Patients with Chronic Prostatitis and Its Clinical Significance

作　　者：魏雪栋[1] 侯建全[1] 张光波[1] 袁和兴[1] 

机构地区：[1]苏州大学附属第一医院泌尿外科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2011年第3期441-443,共3页Suzhou University Journal of Medical Science

基　　金：国家自然科学基金资助项目(30901789;30930085;30771958);教育部博士点基金资助项目(200802850014);江苏省高校自然科学研究基金资助项目(07KJB320107)

摘　　要：目的检测慢性前列腺炎(CP)患者血清中是否存在可溶性B7-H3(sB7-H3),并探讨其临床意义。方法采用酶联免疫吸附法(ELISA)测定79例CP患者血清中sB7-H3水平,并以11名健康男性作为对照。按美国国立卫生研究院(NIH)分类法将79例CP患者分为3型:Ⅱ型9例,ⅢA型21例,ⅢB型49例。患者症状轻重程度均通过慢性前列腺炎症状评分表(CPSI)判定。分析各组间血清中sB7-H3水平差异,以及与前列腺液(EPS)中白细胞(WBC)、卵磷脂小体(SPL)计数间的关系,并进一步分析其与CPSI评分之间的关系。结果对照组与CP组血清中sB7-H3水平分别为(3.685±1.881)ng/ml和(3.619±1.514)ng/ml,两者差异无统计学意义(P>0.05)。但Ⅱ型组(2.305±0.732 ng/ml)明显低于ⅢA型(3.670±0.771 ng/ml)及ⅢB型(3.839±1.736 ng/ml)组(均P<0.01)。从CP患者中诊断Ⅱ型及Ⅲ型患者的ROC曲线下面积为0.814,取最佳诊断点为2.943 ng/ml时,灵敏度为71.4%,特异度为88.9%。血清sB7-H3与CPSI评分、WBC、SPL间无相关关系(P>0.05)。结论血清sB7-H3检测可能有助于区分Ⅱ型及Ⅲ型前列腺炎。Objective To investigate whether soluble B7-H3（sB7-H3） is detectable in the serum of the patients with chronic prostatitis（CP）,and to evaluate the clinical significance of it.Methods Enzyme-linked immunosorbent assays of the serum and EPS for sB7-H3 were done in 90 men： the controls（n=11）,CP（n=79） including chronic bacterial prostatitis（National Institutes of Health category Ⅱ）（n=9）,inflammatory chronic non-bacterial prostatitis/chronic pelvic pain syndrome（CPPS）（NIH category ⅢA）（n=21）,noninflammatory CPPS（NIH category ⅢB）（n=49）.The differences between groups,correlation with white blood cell counts in EPS as well as small particle of lecithin counts in EPS were analyzed.And the results were also correlated with scores for pain,urinary symptoms and quality-of-life impact using a validated symptom index,the National Institutes of Health Chronic Prostatitis Symptom Index（NIH-CPSI）.Results sB7-H3 was detectable in serum of all patients.Mean levels of serum sB7-H3 were similar in the controls（3.685±1.881 ng/ml） and CP（3.619±1.514 ng/ml） groups.The sB7-H3 levels were significantly lower in Ⅱ group（2.305±0.732 ng/ml） than that in ⅢA（3.670±0.771 ng/ml） and ⅢB（3.839±1.736 ng/ml） groups（P0.01）.When receiver operating curve utilizing serum sB7-H3 levels were greater than 2.943 ng/ml for the identified Ⅱ and Ⅲ patients,the sensitivity was 71.4% and specificity 88.9%.But the levels of serum sB7-H3 showed no correlation with NIH-CPSI symptom scores in men with CP.Conclusion The level of sB7-H3 in serum of patients with CP may be a useful marker for discriminating type Ⅲ from type Ⅱ.

关 键 词：慢性前列腺炎 B7-H3 慢性前列腺炎症状评分 白细胞 卵磷脂小体 

分 类 号：R697.33[医药卫生—泌尿科学]