慢性肾脏病患者肾脏vWF和ADAMTS13表达改变及其意义  

Von Willebrand Factor and ADAMTS13 Expression in Renal Tissue in Chronic Glomerulonephritis Diseases

作　　者：沈蕾[1] 卢国元[1] 蒋丽琼[1] 董宁征[2] 乔青[1] 李明[1] 阮长耿[2] 

机构地区：[1]苏州大学附属第一医院肾内科,江苏苏州215006 [2]苏州大学附属第一医院江苏省血液研究所卫生部血栓与止血重点实验室,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2011年第3期451-453,485,共4页Suzhou University Journal of Medical Science

基　　金：2009年苏州大学医学部研究生创新基金

摘　　要：目的观察血管性血友病因子(vWF)及其裂解酶ADAMTS13在慢性肾脏病不同病理类型中的表达以及与临床指标的相关性。方法采用免疫组织化学分析vWF和ADAMTS13在36例不同病理类型慢性肾炎患者肾脏中的表达,Image J图象分析软件测定vWF和ADAMTS13的阳性染色面积的比例。结果 (1)肾病综合征患者肾小球阳性染色面积vWF/ADAMTS13明显高于慢性肾炎组(P<0.05);(2)膜性肾病组ADAMTS13表达明显低于非膜性肾病组(P<0.05);(3)vWF/ADAMTS13阳性染色面积比与蛋白尿呈正相关(r=0.512,P<0.01)。结论慢性肾脏病局部vWF和ADAMTS13表达间平衡失调与慢性肾脏病的临床和病理类型有关,能在一定程度上反映肾脏微循环的变化。Objective To observe the expression of von Willebrand factor（vWF） and its cleaving protease——ADAMTS13 in renal tissues of patients with different pathological and clinical types of chronic kidney diseases.Methods Thirty six cases of chronic kidney disease＇patients were involved in the study.Expression of vWF and ADAMTS13 in renal tissues was analyzed by immunohistochemistry.Image J analysis software was used to measure vWF and ADAMTS13 expression.The results were expressed as positive staining area percentage of total glomerular.Results（1） The vWF/ADAMTS13 positive staining eara in the NS group was significantly higher than that in the CGN group（P0.05）.（2） ADAMTS13 positive staining eara was significantly lower in MN group than that in the non-MN group（P0.05）.（3） vWF/ADAMTS13 expression level was positively correlated with proteinuria（r=0.512,P0.01）.Conclusion The imbalance of local expression of vWF and ADAMTS13 in chronic kidney disease is related to the clinical and pathological type in a certain extent,may reflect changes in renal microcirculation in some degree.

关 键 词：血管性血友病因子 ADAMTS13 免疫组化 慢性肾脏病 

分 类 号：R692[医药卫生—泌尿科学]