黑斑病对甘薯体内几种保护酶活性的影响  

Effects of Black Rot on several protective enzymes activity in sweet potato

作　　者：孙厚俊[1] 刘美艳[1,2] 宗玮玮[2] 赵永强[1] 陈晓宇[1] 谢逸萍[1] 

机构地区：[1]中国农科院甘薯研究所,江苏徐州市221121 [2]徐州师范大学,江苏徐州市221116

出　　处：《广西农学报》2011年第3期14-16,19,共4页Journal of Guangxi Agriculture

基　　金：国家现代农业产业技术体系(nycytx-16-B-9)

摘　　要：通过对甘薯抗病品种南京92和感病品种烟252感染黑斑病后三种保护酶活性的测定,了解不同抗性甘薯品种块根感病后POD、SOD和CAT的变化,探明甘薯品种抗黑斑病生理生化机制。结果表明,接种黑斑病菌后,各处理的过氧化物酶(POD)和超氧化物歧化酶(SOD)活性均显著升高,且抗病品种的酶活变化速度和峰值均高于感病品种,抗病品种南京92第4d POD、SOD活性值分别达536U/min.mg、856U/gFw;感病品种第4d POD、SOD活性值分别达348U/min.mg、568U/gFw;抗感品种过氧化氢酶(CAT)活性均显著降低,抗感品种的下降幅度分别为73.17%、63.64%。POD、SOD和CAT的活性的变化表明,甘薯品种的酶活变化与黑斑病抗性存在相关性,且抗感品种的酶活变化有一定的规律。In order to reveal the physiological and biochemical mechanism of black rot resistance,the activity of POD,SOD and CAT in resistant and susceptible sweet potato treated with black rot pathogen was tested.The results showed that inoculation of black rot significantly enhanced the activity of SOD and POD in black rot resistant cultivar,Nanjing-92,and susceptible cultivar,Yan-252.Both SOD and POD activity in Nanjing-92 treated with black rot was significantly higher than in treated Yan-252.The activity of SOD and POD peaked in the 4th day after inoculation,at 536U/min·mg and 856U/gFw in Nanjing-92,and 348U/min·mg and 568U/gFw in Yan-252.The CAT activity was reduced after inoculation to 73.17% in Nanjing-92 and 63.63% in Yan-252.The experimental results suggested that the change of POD,SOD and CAT activity induced by inoculation of black rot is connected to the black rot resistance of sweet potato.

关 键 词：甘薯 甘薯黑斑病 酶活性 抗病机制 

分 类 号：S435.31[农业科学—农业昆虫与害虫防治]