日本血吸虫感染鼠巨噬细胞IL-13Rα2的表达  

Expression on IL-13Rα2 in macrophages of Schistosoma japonicum-infected mice

作　　者：王维[1] 郑胜生[1] 祁瑶[1] 闻惠琴[1] 刘丽丽[1] 沈继龙[1] 

机构地区：[1]安徽医科大学病原生物学教研室,人畜共患病安徽省重点实验室,合肥230032

出　　处：《中国人兽共患病学报》2011年第7期597-600,609,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学主任基金项目(30940061);安徽省高等学校省级自然科学研究计划重点项目(KJ2009A80);安徽医科大学博士启动基金(XJ201012)联合资助

摘　　要：目的检测IL-13的诱骗受体IL-13Rα2在血吸虫病巨噬细胞的表达,为从细胞水平探讨Th2型免疫性疾病分子病理机制奠定基础。方法建立日本血吸虫病鼠模型,采用免疫荧光标记法分别检测肝肉芽肿和原代巨噬细胞IL-13Rα2蛋白的表达情况。结果荧光显微镜下观察:肝肉芽肿内可见似"咖啡豆样"散在分布的IL-13Rα2免疫阳性信号,巨噬细胞膜内缘呈异常增强的环状荧光。结论巨噬细胞是IL-13Rα2阳性表达细胞,IL-13Rα2是血吸虫病重要的免疫病理调节分子。The purpose of this study was to investigate the expression on intracellular proteins of IL-13Rα2 in the macrophages of Schistosomiasis japonicum mice,and to offer the experiment foundation for elucidating IL-13 responding cell and mechanism of fibrogenesis in Th2-mediated diseases.A large number of mice were exposed to cercariae and established S.japonicum mouse model.IL-13Rα2 expressed in the granulomas of liver tissues and peritoneal macrophages was determined by immunofluorescent labeling with primary antibody of goat anti-mouse IL-13Rα2 antibody and monoclonal anti-CD68 antibody,respectively.Coffee bean-like IL-13Rα2 positive staining was showed in the hepatic granuloma in fluorescentmicroscopy.Co-expression of IL-13Rα2 and CD68 protein in peritoneal macrophages from 8-week infected mice was observed.It exhibited the distribution of IL-13Rα2 in cytoplasm concentrated on the inner layer of membrane in macrophages.It＇s suggested that IL-13Rα2 expressing macrophages might be a critical contributor to the immunopathogenesis of schistosomiasis.

关 键 词：血吸虫病 肉芽肿 巨噬细胞 IL-13RΑ2 CD68 免疫荧光染色技术 

分 类 号：R383.2[医药卫生—医学寄生虫学]