DIGFA-kit和IEST检测人、鼠斯氏狸殖吸虫抗体的研究  

Detection of Pagumogonimus skrjabini antibody with DIGFA-kit and IEST

作　　者：朱名胜[1] 朱敬[2] 王绍基[1] 宋明华[1] 

机构地区：[1]湖北医药学院寄生虫学教研室,十堰442000 [2]湖北医药学院生物学教研室

出　　处：《中国人兽共患病学报》2011年第7期645-646,652,共3页Chinese Journal of Zoonoses

基　　金：日本健康科学基金项目资助(JHSF;HS189)

摘　　要：目的探索用肺吸虫病金标渗滤试剂盒(DIGFA-kit)和免疫酶染色试验(IEST)检测斯氏狸殖吸虫抗体的敏感性和特异性。方法采用DIGFA-kit和IEST检测流行区人群和病鼠血清抗体。结果斯氏狸殖吸虫病流行区人群和病鼠血清特异性抗体两种方法均为5.08%和100%。用此二种血清学方法检测正常大鼠、旋毛虫病大鼠、血吸虫病兔和蛔虫病人血清,除1例血吸虫病兔血清DIGFA-kit出现阳性反应外,其它血清两种方法均为阴性反应。实验动物从感染后第2周两种方法开始抗体检测阳性,第4周阳性率均达100%,持续至8周。结论二种方法对斯氏狸殖吸虫特异性IgG抗体的检测均有较好的敏感性和特异性。The sensitivity and specificity of dot immunogold filtration asser（DIGFA） and IEST was explored in this study for detection of Pagumogonimus skrjabini antibody.The P.skrjabini antibody in sera from human and infected rats in endemic area were tested by using DIGFA and IEST methods.The positive rats of the antibody in sera from human and infected rats were 5.08% and 100% respectively.In the detection results of health big rats,big rats with trichinosis,rabbits with schistosomiasis,and human ascariasis cases,only one was positive with DIGFA in rabbits with schistosomiasis.The others in control group were negative.The antibody was detected out at 2 weeks post-infection in experimental animal,and at 4 weeks the positive rate reached 100% and lasted out 8 weeks.The results show that these two methods have good sensitivity and specificity in detection of Pagumogonimus skrjabini antibodies.

关 键 词：斯氏狸殖吸虫 抗体 血清学试验 

分 类 号：R383.2[医药卫生—医学寄生虫学]