我国恒河猴粪便腺病毒聚合酶基因片段的序列分析  

Sequencing and analyses of the adenovirus polymerase gene in fecal samples of captively bred rhesus macaques in China

作　　者：卢建溪[1] 钱师宇[2] 王强[1] 阳莉[1] 李刚[3] 

机构地区：[1]中山大学附属第三医院疫苗研究所,广州510630 [2]暨南大学医学院医学信息教研室,广州510632 [3]中山大学附属第三医院感染科,广州510630

出　　处：《中国人兽共患病学报》2011年第8期700-703,共4页Chinese Journal of Zoonoses

基　　金：国家863计划(No.2007AA021002);广东省科技计划资助项目(No.2008B030301101)资助

摘　　要：目的研究非人灵长类动物猴子腺病毒的分子多样性。方法采集圈养的恒河猴粪便样本,以腺病毒聚合酶基因为目的基因,用PCR方法进行扩增,对PCR阳性产物进行克隆、测序,并进行系统进化分析。结果 57只恒河猴粪便样本有12份存在腺病毒DNA,系统进化分析提示这些序列主要可分为两大组:类SAdV-6组(2个非重复序列)和类SAdV-7组(9个非重复序列)。此外,有3个克隆,其最相似序列分别为:SAdV-1,SAdV-3和HAdV-52。结论研究证实腺病毒在非人灵长类粪便中普遍存在,揭示这些动物肠道内腺病毒的多样性以及系统进化情况。In an attempt to study the moleculr characterization and epidemiology of simian adenoviruses in nonhuman primate （NHP） populations. We examined a colony of captively bred rhesus macaques （Macaca mulatta） in China for the presence of adenoviral DNA in stool samples. This was done by using the PCR method that targeted the adenovirus polymerase gene, and the PCR positive fragments were cloned for sequencing and phylogenetic analyses. Among the 57 animals analyzed, fecal samples from 12 animals were positive for the presence of adenoviral DNA . The results suggested that the viral DNA clones were primarily segregated into two large groups： SAdV-6 （2 non redundant sequences） and SAdV-7 （9 non-redundant se quences）. In addition, there were three clones with more similarity to SAdV-1, SAdV-3 and HAdV-52 respectively. Our data confirmed the prevalence of adenoviral DNA in the feces of NHPs and revealed the heterogeneity and phylogenetics of the adenoviruses in the gastrointestinal tract of the study animals.

关 键 词：恒河猴 猴子腺病毒 基因组多样性 

分 类 号：R373[医药卫生—病原生物学]