慢性乙型肝炎肝纤维化患者外周血CD4^+CD25^+CD127^(low/-) T细胞与人肝星状细胞共培养的实验研究  

The Study of Co-culturing CD4^+CD25^+CD127^(low/-) T Cells from Patients with HBV-related Liver Fibrosis and Hepatic Stellate Cells in vitro.

作　　者：金晓芝[1] 陈永平[1] 程瑗[1] 潘黎静[2] 潘陈为[3] 戴志娟[1] 叶超[1] 

机构地区：[1]浙江省温州医学院附属第一医院感染内科 [2]温州市第二人民医院病理科 [3]浙江省温州医学院附属第二医院感染内科

出　　处：《医学研究杂志》2011年第8期70-73,共4页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(Y207464)

摘　　要：目的探讨CD4+CD25+CD127low/-T细胞在慢性乙型病毒性肝炎肝纤维化发病机制中的作用。方法按肝纤维化S分期将慢性乙型肝炎肝纤维化患者分为S1~S2期6例和S3~S4期7例;采用磁激活细胞分选法(MACS)提取13例患者和6例健康对照者外周血CD4+CD25+CD127low/-T细胞;分别将CD4+CD25+CD127low/-T细胞以不同比例与人肝星状细胞株(hepaticstellate cell,HSC)共培养5天,检测各组HSC的增殖及TGF-β1的分泌。结果 CD4+CD25+CD127low/-T细胞与HSC之比,当健康对照组和S1~S2期组为1.5∶1,S3~S4期组为2∶1时,HSC的增殖速度和TGF-β1分泌量均在同组不同比例中达最高值。各组同比例之间,当比例为1.5∶1时,健康对照组HSC的增殖速度显著高于S3~S4期组(P<0.05);在比例为1∶1~2∶1范围内,S1~S2期组和S3~S4期组HSC的TGF-β1分泌量远高于同比例健康对照组(P<0.05)。结论 CD4+CD25+CD127low/-T细胞可能通过自身细胞数量和功能的双重调节,影响肝星状细胞的增殖和分泌,参与了慢乙肝肝纤维化的进展。Objective To investigate the roles of CD4＋CD25＋CD127^low/-T cells in HBV - related liver fibrosis. Methods Fibro- sis stage was confirmed by pathohistological examination of liver section. CD4＋CD25＋CD127^low/-T cells were isolated respectively from peripheral blood of 6 healths,6 patients in S1 - S2 stage and 7 patients in S3 - S4 stage by MACS. We then cultured CD4＋CD25＋CD127^low/-T cells and human hepatic stellate cell（HSC） with different ratios respectively,and after 5 days,appraised the proliferation of HSC by CCK8 and used Elisa method to test concentration of TGF - β1 secreted by HSC. Results We discovered the strongest prolifera- tion and the highest TGF - β1 secretion of HSC when the healths and patients in S1 - S2 stage ratio of （the numbers of CD4＋CD25＋CD127^low/-T cells/HSC） 1.5：1 ,patients in S3 - S4 stage of 2： 1. From health control group,we also found that HSC had a stronger proliferation compared with patients in S3 - S4 stage when ratio was 1.5：1 （P 〈 0. 05 ）. And the ratios ranged from 1：1 to 2： 1. There was a lower TGF -β1 secretion of HSC in health control group compared with other groups（P 〈 0.05）. Conclusion Changes of numbers and function- alities of CD4＋CD25＋CD127^low/-T cells might promote HSC proliferation and TGF - β1 secretion and contribute to progression of HBV - related liver fibrosis.

关 键 词：慢性乙型病毒性肝炎 肝纤维化 CD4+CD25+CD127low/- Treg MACS 

分 类 号：R512.62[医药卫生—内科学]