少弱精子症患者精浆中精子及睾丸组织端粒酶活性实验研究  

Experimental study for telomerase activity in patients with oligoasthenozoospermia

作　　者：杜晓钟[1] 陕文生[1] 郑雷[1] 

机构地区：[1]甘肃省妇幼保健院检验科,兰州730050

出　　处：《国际检验医学杂志》2011年第12期1277-1278,共2页International Journal of Laboratory Medicine

基　　金：甘肃省自然科学基金资助项目(ZS031-A25-065-E)

摘　　要：目的通过对少弱精子症患者精浆及睾丸端粒酶活性(TA)的检测,探讨少弱精子症患者精浆中精子与TA的相关性。方法以端粒重复序列扩增法(TRAP)检测受试者精浆中精子和睾丸组织TA。结果少弱精子症患者精子TA表达与健康者比较差异无统计学意义(P>0.05);少弱精子症患者睾丸组织中TA呈阳性。结论睾丸组织的TA是反映精子形成的高敏感和高特异标记物;健康者和少弱精子症患者精浆TA降低,TA的缺乏可能是生殖细胞发育停滞的原因之一。Objective To investigate the relationship between telomerase activity（TA）and the sperms in seminal plasma of patients with oligoasthenozoospermia by the detection of the TA of seminal plasma and testis samples.Methods Telomeric repeat amplification protocol（TRAP）was performed for the detection of the TA of seminal plasma and testis samples of all subjects.Results There was no statistical difference of the expression of TA in sperm samples between patients with oligoasthenozoospermia and healthy controls.Testis samples from patients with oligoasthenozoospermia were positive with TA.Conclusion TA in testicular tissues could be highly sensitive and specific marker for the formation of sperms.It was confirmed of the decrease of the level of TA in seminal plasma of healthy individuals and patients with oligoasthenozoospermia,which might be one of the reasons,causing the arrest of development of germ cells

关 键 词：弱精子症 端粒酶活性 端粒重复序列扩增 实验研究 

分 类 号：R698.2[医药卫生—泌尿科学]