臭鼻克雷伯和鲍曼不动杆菌中检出NDM-1型金属β内酰胺酶基因  

Detecttion of New Delhi metallo-β-lactamase 1 gene in Klebsiella ozaenae and Acinetobacter baumannii

作　　者：杨银梅[1] 叶惠芬[1] 张伟红[1] 陈惠玲[1] 周小棉[1] 

机构地区：[1]广州医学院附属广州市第一人民医院检验科,510180

出　　处：《国际检验医学杂志》2011年第13期1407-1409,共3页International Journal of Laboratory Medicine

基　　金："十一五"艾滋病和病毒性肝炎等重大传染病防治专项资助项目(2008ZX10004-004-1);国家自然基金资助项目(30870753);广东省自然科学基金资助项目(9151008901000190);广东省医学科研基金资助项目(A2009518);广州市医药卫生科技基金重点资助项目(2009-ZDi-10);广州市科技支撑计划资助项目(2010J-E241-2)

摘　　要：目的调查对碳青霉烯类抗菌剂敏感性降低的肠杆菌和鲍曼不动杆菌中NDM-1型金属β内酰胺酶基因的存在情况。方法应用改良Hodge试验和EDTA协同试验对2007～2010年本院收集的对碳青霉烯类抗菌剂敏感性降低的肠杆菌和鲍曼不动杆菌进行碳青霉烯酶筛选。PCR法扩增NDM-1基因,并对PCR扩增阳性产物进行DNA测序分析。对NDM-1基因阳性菌株用PCR方法检测armA和rmtB16S rRNA甲基化酶基因,VIM、SPM、IMP和KPC碳青霉烯酶基因以及Ⅰ类整合子。Etests法进行药敏试验。结果 70株肠杆菌中10株Hodge Test和EDTA协同试验阳性,55株鲍曼不动杆菌中6株EDTA协同试验阳性。其中1株臭鼻克雷伯和3株鲍曼不动杆菌PCR扩增出NDM-1型金属β内酰胺酶基因目的片段,经测序比对为NDM-1型基因。其中1株臭鼻克雷伯和1株鲍曼不动杆菌检测出armA型16S rRNA甲基化酶基因,1株鲍曼不动杆菌检测出2000 bp左右的Ⅰ类整合子,未检出除NDM-1型外的其他碳青霉烯酶基因。NDM-1基因阳性菌除对替加环素、多黏菌素B敏感外,对其他检测的抗菌剂均耐药。结论臭鼻克雷伯和鲍曼不动杆菌中存在NDM-1型金属β内酰胺酶基因,有的携带armA型16S rRNA甲基化酶基因和Ⅰ类整合子,为该地区首次报道。Objective To investigate the prevalence of NDM-1 gene in multidrug-resistant Enterobacteriaceae and Acinetobacter baumannii.Methods Carbapenemases were screened by Modified Hodge Test and EDTA-disk synergy test.The encoding genes of NDM-1 was amplified by PCR and DNA sequencing.Encoding genes of 16S rRNA methylase,VIM,SPM,IMP and KPC gene was amplified by PCR and DNA sequencing.Antibiotic susceptibilities were assessed by Etests.Results 10 of 70 isolates Enterobacteriaceae were positive by Hodge Test and EDTA-disk synergy test,6 of 55 isolates Acinetobacter baumannii were positive by EDTA-disk synergy test.Of these 125 Enterobacteriaceae and Acinetobacter baumannii,1 Klebsiella ozaenae and 3 Acinetobacter baumannii were NDM-1 positive by PCR and DNA sequencing.1 Klebsiella ozaenae and 1 Acinetobacter baumannii were armA 16S rRNA methylase gene positive.the strain carrying NDM-1 gene was sensitive to polymyxin B and tigecycline.Conclusion Klebsiella ozaenae and Acinetobacter baumannii carry NDM-1 gene,This is the first report of NDM-1 in Guangzhou.

关 键 词：碳青霉烯酶 NDM-1型金属β内酰胺酶基因 抗药性 细菌 革兰氏阴性菌 

分 类 号：R440[医药卫生—诊断学]