左氧氟沙星印迹聚合物微球及其特异吸附研究  

Study on the synthesis and characteristics of levofloxacin molecularly imprinted polymer microsphere

作　　者：高燕哺[1] 朱桂芬[1] 樊静[1] 

机构地区：[1]河南师范大学化学与环境科学学院,黄淮水环境与污染防治省部共建教育部重点实验室,河南省环境污染控制重点实验室,河南新乡453007

出　　处：《化学研究与应用》2011年第8期980-984,共5页Chemical Research and Application

基　　金：教育部博士点基金(200804760004)资助;河南省基础与前沿技术研究计划(082300423202)资助;(102300410194)资助

摘　　要：以甲基丙烯酸为功能单体,乙二醇二甲基丙烯酸酯为交联剂,采用沉淀聚合法制备了左氧氟沙星印迹聚合物(MIP)微球,用SEM和IR分别对微球形貌和结构进行表征,用静态吸附法和Scatchard分析考察了聚合物微球对模板及其结构类似物的吸附行为和选择性识别能力。结果表明,MIP对模板分子的吸附平衡速度快,40 min即可达到吸附平衡。吸附特征符合假二级动力学方程;Scatchard分析表明,MIP对底物有两类结合位点,其离解常数分别为0.672 mmol/L和1.541 mmol/L,两类结合位点的饱和吸附量分别为313μmol/g和378μmol/g。本法制备的MIP微球对结构类似的沙星类抗生素的选择性吸附能力优于化学组成相同的非印迹聚合物微球。A molecularly imprinted polymer（MIP）microsphere using levofloxacin as template molecule,MAA as function monomer,EDMA as cross-linking agent was synthesized via precipitation polymerization.The surface morphologies and characteristics of the microsphere were characterized by infrared spectroscopy and scanning electron microscopy.The adsorption properties and selective adsorption capacity of polymer microsphere for the template and the anologues were determined by the static adsorption equilibrium experiments and the Scatchard analysis.The results showed that the adsorption was fast and the adsorption equilibrium was achieved in 40 min.The adsorption process of MIP upon template could be described by a pseudo-second-order kinetics.There were two kinds of binding sites in the MIP and the dissociation constants were 0.672 mmol/L and 1.541 mmol/L,respectively.The maximum adsorption capacities of the two kinds of binding sites were 313 μmol/g and 378 μmol/g,respectively.Compared with the corresponding non-imprinted polymer,MIP exhibited better adsorption properties and selectivity for the template and the anologues.

关 键 词：沉淀聚合 左氧氟沙星 分子印迹聚合物 特异吸附 

分 类 号：O647.33[理学—物理化学]