2005年河南省局部地区伤寒副伤寒沙门菌株脉冲场凝胶电泳分型研究  

Henan Province in 2005 typhoid and paratyphoid monk strain pulse field gel electrophoresis member minute

作　　者：王建丽[1] 马宏[1] 许玉玲[1] 黄丽莉[1] 郭大城[1] 张爱梅[1] 张保华[1] 

机构地区：[1]河南省疾病预防控制中心,河南郑州450016

出　　处：《河南预防医学杂志》2011年第4期246-248,共3页Henan Journal of Preventive Medicine

摘　　要：目的了解河南省伤寒、副伤寒沙门菌主要流行菌型。方法用脉冲场凝胶电泳(PFGE)进行分型,NTSYSpc 2.1软件聚类分析。结果来自河南省两个监测点的伤寒、副伤寒沙门菌6株分离菌,根据XbaI酶切PFGE基因指纹图谱分析6,株菌出现5个PFGE基因型别:甲、乙副伤寒沙门菌,伤寒沙门菌3个PFGE型别。结论明确了2005年河南局部伤寒沙门菌流行菌型为X1、X2、X3三个基因型别,副伤寒沙门菌流行菌型有X4、X5基因型。Objection To investigate the main epidemic serotype of typhoid and paratyphoid Salmonella of Henan Province.Methods Pulsed field gel electrophoresis（PFGE）was used in the molecular typing.Results were analyzed with NTSYSpc 2.1 software for the cluster analysis.Results The six isolates of typhoid and paratyphoid Salmonella collected from the patients in two monitoring sites of Henan province could be divided into 5 PFGE types（X1～X5）,based on the size and number of restriction fragments and patterns,that were digested by XbaⅠ.In addition to A,B and Salmonella,the Salmonella typhi had three PFGE type.Conclusion Data identified that there were five epidemic serotype,X1,X2,X3 genotype in Salmonella and X4,X5 genotype in paratyphoid salmonella after 2005 year of Henan Province.

关 键 词：脉冲场凝胶电泳 伤寒 副伤寒沙门菌 分子分型 

分 类 号：R516.3[医药卫生—内科学]