盲肠结扎穿孔脓毒症大鼠巨噬细胞炎症蛋白-1α水平变化的研究  

Serum macrophage inflammatory protein-1 αlevel in septic rats

作　　者：童飞[1] 胡德林[1] 余又新[1] 王春华[1] 方林森[1] 徐庆连[1] 

机构地区：[1]安徽医科大学第一附属医院烧伤科,安徽合肥230022

出　　处：《安徽医药》2011年第9期1099-1100,共2页Anhui Medical and Pharmaceutical Journal

基　　金：2009年安徽省卫生厅科研基金资助(No09A067)

摘　　要：目的研究脓毒症大鼠早期肺泡灌洗液巨噬细胞炎症蛋白-1α(MIP-1α)水平的变化及其与脓毒症的关系。方法 60只SD大鼠随机分成A组假手术组,n=30、B组脓毒症组,n=30,A组仅行盲肠探查术,B组采用盲肠结扎穿孔(CLP)制作大鼠脓毒症模型。两组术后即刻皮下注射生理盐水5 ml。各组均于术后3、6、12、24、48 h时点,留取肺泡灌洗液检测MIP-1α。结果 CLP术后3 h肺泡灌洗液MIP-1α即开始升高,明显高于A组(P<0.05),且逐步升高,到术后12 h达峰值,随后逐渐下降,但在48 h时点仍然显著高于A组(P<0.05)。结论脓毒症致急性肺损伤后MIP-1α水平明显升高,其升高可能是脓毒症导致急性肺损伤的原因之一,在脓毒症导致急性肺损伤发生发展中具有重要作用。Aim To study septic rats on changes in macrophage inflammatory protein-MIP-1α level in serum.Methods 60 SD rats were divided into two groups randomly：group A sham group,n=30 and group B sepsis group,n=30.In group A,appendix exploration was made while in group B,the animals received cecal ligation and puncture CLP.In each group,5 ml of saline was subcutaneously injected saline of 5 ml.All rats were taken to measure the levels of macrophage inflammatory protein-1α in serum through bronchial alvolar lavage fluid at 3,6,12,24,48 h.Results Compared with group A,the levels of MIP-1α in serum were increased significantly in group B（P0.05）.Conclusion The high levels of MIP-1α may be the causes of the damaged microcirculation and the elevated vascular may have a certain effect on the sepsis.

关 键 词：脓毒症 巨噬细胞炎症蛋白-1Α 

分 类 号：R459.7[医药卫生—急诊医学]