EIAV强毒株和疫苗株gp45的纯化及结晶比较  

Purification and Crystallization Comparison between EIAV gp45 of Virulent Strain and Vaccine Strain

作　　者：王建新[1] 王敏[1] 刘新奇[2] 

机构地区：[1]天津科技大学生物工程学院,天津300457 [2]南开大学生命科学学院,天津300071

出　　处：《天津科技大学学报》2011年第4期10-13,25,共5页Journal of Tianjin University of Science & Technology

基　　金：国家973计划项目资助(2010CB911800);"艾滋病和病毒性肝炎等重大传染病防治"专项(2008ZX10001-010)

摘　　要：EIAV(equine infectious anemia virus)疫苗是世界唯一成功的慢病毒疫苗.通过对强毒株和疫苗株EIAV相关蛋白结构的比较研究,可以促进对其致弱过程及免疫机制的了解.gp45(glycoprotein 45)是EIAV介导与宿主膜融合过程的关键蛋白.以EIAV强毒株LN40和疫苗株FDDV13为研究对象,对gp,45核心结构域进行了克隆、表达,并对纯化和晶体生长进行了比较,为进一步的三维结构解析,更好地理解EIAV减毒疫苗的免疫机制及相关疫苗设计奠定了基础.EIAV （equine infectious anemia virus） vaccine is the only successful lentivirus vaccine in the world. By comparison studies of EIAV virulent strain and vaccine strain related protein structures, better realization of the attenuation process and immune mechanism can be achieved. The gp45 （glycoprotein 45） is a key protein in mediating membrane fusion between EIAV and host cell. Taking EIAV virulent strain LN40 and vaccine strain FDDV13 as research subjects, the core structure of virulent strain and vaccine strain gp45 was cloned, expressed, and purification and crystallization characters were compared, which laid the foundation for further resolution of the three dimensional structure, better understanding of immune mechanism of EIAV attenuated vaccine and related vaccine design.

关 键 词：EIAV 强毒株 疫苗株 gp45 结晶 

分 类 号：Q518.2[生物学—生物化学]