调肝方药对愤怒和郁怒情绪模型大鼠下丘脑5-羟色胺3B受体表达的影响  

Effects of Chinese herbal medicines for regulating liver qi on expression of 5-hydroxytryptamine 3B receptor in hypothalamic tissues of rats with anger emotion

作　　者：葛庆芳[1] 张惠云[1] 

机构地区：[1]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355

出　　处：《中西医结合学报》2011年第8期871-877,共7页Journal of Chinese Integrative Medicine

基　　金：国家重点基础研究发展计划(973计划)资助课题(No.2011CB505102);国家自然科学基金重点资助项目(No.30930110);国家自然科学基金面上资助项目(No.30973688);山东省科技发展计划资助项目(No.2010GSF10290)

摘　　要：目的:观察大鼠下丘脑5-羟色胺3B受体(5-hydroxytryptamine 3 Breceptor,5-HT3BR)表达的变化,探讨大鼠下丘脑5-HT3BR在愤怒和郁怒反应情绪发生机制中的作用,研究中药复方经前平颗粒和经前舒颗粒改善愤怒和郁怒情绪的中枢作用靶点。方法:采用居住入侵结合社会隔离的方法分别复制愤怒和郁怒情绪模型大鼠。两种模型给药组分别给予经前平、经前舒颗粒干预,模型组和正常组给予灭菌水;以旷场实验和糖水偏好系数测试评价模型,并利用逆转录-聚合酶链反应和蛋白质印迹法检测各组大鼠下丘脑5-HT3BR的mRNA水平和蛋白水平的表达。结果:与正常组比较,郁怒模型组大鼠下丘脑5-HT3BR的mRNA和蛋白表达都明显增高(P<0.01),愤怒模型组显著下降(P<0.01);与模型组比较,经前平和经前舒颗粒可改善5-HT3BR的mRNA和蛋白的表达(P<0.01),且与正常组的差异无统计学意义。结论:郁怒大鼠下丘脑5-HT3BR表达升高,而愤怒大鼠5-HT3BR表达下降。调肝方药经前平和经前舒颗粒可能通过调节下丘脑5-HT3BR的mRNA和蛋白的表达水平调节愤怒或郁怒情绪。Objective：To explore the central mechanisms of anger emotion and the effects of Chinese herbal medicines for regulating liver qi on the anger emotion and the expression level of 5-hydroxytryptamine 3B receptor（5-HT3BR）in rat hypothalamus.Methods：Rat models of anger-in or anger-out emotions were prepared by the methods of resident intruder paradigm.There were five groups in this study：control,anger-in model,Jingqianshu Granule-treated anger-in,anger-out model and Jingqianping Granule-treated anger-out groups.The treatment groups were orally given Jingqianshu granules and Jingqianping granules respectively,and the model groups and the normal control group were given sterile water.Open-field test and sucrose preference test were used to evaluate behavioristics of the rats.Semi-quantitative reverse transcription-polymerase chain reaction and Western blot methods were used to detect the expression levels of 5-HT3BR mRNA and protein in the rat hypothalamus.Results：The expression of 5-HT3BR in hypothalamus of anger-in model rats increased obviously（P〈0.01）and that of anger-out model rats decreased obviously（P〈0.01）compared with the normal control group.Compared with the model group,the expressions of 5-HT3BR in the treatment groups were significantly improved（P〈0.01）after treatment,and recovered to normal level.Conclusion：The anger-in stimulation obviously increases hypothalamic 5-HT3BR expression and the anger-out emotion can obviously reduce its expression.Chinese herbal medicines for regulating liver qi may treat anger emotion in rats by improving the hypothalamic 5-HT3BR protein and gene expression levels.

关 键 词：愤怒 郁怒 下丘脑 5-羟色胺3B受体 中草药 大鼠 

分 类 号：R285.5[医药卫生—中药学]