手性膦酸锆/血红蛋白/金电极的安培型过氧化氢传感器的研究  

Amperometric Hydrogen Peroxide Biosensor Based on Electro-Codeposition of Chiral Zirconium Phosphonate/Hemoglobin on Gold Electrode

作　　者：吴宗方[1] 刘昌华[1] 藤瑛丽[1] 徐静[1] 

机构地区：[1]西南大学化学化工学院,重庆400715

出　　处：《西南大学学报（自然科学版）》2011年第5期50-54,共5页Journal of Southwest University(Natural Science Edition)

摘　　要：共电沉积手性膦酸锆Zr(PO4)(H2PO4)0.50(HO3PCH2NCH2SC2H3COOH)0.50.1·6 H2O(ZrPMT)、血红蛋白(Hb)于金电极,制备了安培型过氧化氢生物传感器.制得的生物传感器对于过氧化氢(H2O2)有着良好的响应,检出限为1.6×10-7mol/L,线性范围为5×10-7～2.2×10-4mol/L,相关系数0.99(S/N=3),且该传感器有良好的稳定性和较强的抗干扰能力.A new kind of material-chiral zirconium phosphonate（Zr（PO4）（H2PO4）0.50（HO3PCH2NCH2SC2H3COOH）0.50·1.6 H2O）,abbreviated as ZrPMT,was employed for the construction of a hydrogen peroxide biosensor.UV-Vis absorption spectra displayed that hemoglobin（Hb） retained its native structure after interaction with ZrPMT,showing that ZrPMT provided good compatibility.Cyclic voltammetry showed that ZrPMT could greatly improve the electron transformation between the modified electrode and the electrolyte.The biosensor was prepared by electro-codepositing of ZrPMT and Hb onto the gold electrode（Au）.This method was simple and easy,and the biosensor exhibited good response to the reduction of hydrogen peroxide with a linear range from 5×10-7 mol/L to 2.2×10-4 mol/L and a detection limit of 1.6×10-7 mol/L,the correlation coefficient being 0.99（S/N=3）.Its stability and selectivity were also good.

关 键 词：手性膦酸锆 生物传感器 共电沉积 血红蛋白 

分 类 号：O657.15[理学—分析化学]