采用FTIR和电子能谱EDS指纹图谱直观比对鉴定右归丸济生肾气丸  

Identification of Yougui and Jisheng Shenqi Pills with FTIR and EDS Fingerprint Spectra by New Visual Comparison

作　　者：王丽双[1] 邹华彬[1] 田芳[1] 杜爱琴[1] 董凤娟[1] 张新玲[1] 

机构地区：[1]山东大学化学化工学院,山东济南250100

出　　处：《光谱学与光谱分析》2011年第9期2407-2411,共5页Spectroscopy and Spectral Analysis

基　　金：山东省科技攻关计划项目(2009RKA063)资助

摘　　要：结合FTIR和电子能谱EDS指纹图谱,鉴别了两种组成相近的中药复方右归丸和济生肾气丸。测定右归丸和济生肾气丸样品氯仿提取物的红外指纹图谱,依据W检验理论判别方法精细建立两种中药复方红外指纹图谱的特征吸收峰组,确定了各个特征吸收峰相对应的特征分子基团和药物成分组成。同时测量两种中药复方原药粉末样品的EDS指纹图谱。根据两种中药复方FITR指纹图谱中的特征峰组及其对应的化合物类型和原药粉末的EDS谱中多种元素的种类和含量信息,可以快速准确鉴别右归丸和济生肾气丸,使红外指纹图谱法达到了与色谱指纹图谱法相似的鉴别能力。本文建立的FTIR和EDS指纹图谱直观比对法是一种快速准确鉴别组成复杂成分相似的同剂型中药复方的有效方法。FTIR combined with EDS fingerprint spectra was first applied to the identification of two kind of traditional Chinese compound formulae-Yougui pill and Jisheng shenqi pills, which have the similar composition. The IR FPS of extraction of two kinds of pills extracted with chloroform were measured by liquid membrane method. The exclusively characteristic peak groups of these two kinds of formulae were theoretically established based on the Shapiro-Wilk W testing method,and the characteristic radicals and compound species corresponding to each characteristic peak were determined. Meanwhile, EDS fingerprint spectra of the two kinds of original powders were also measured which can reflect the element species and content information. Based on the three kinds of information （characteristic peak groups, radicals and compound species, different elements）, Yougui and Jisheng shenqi pills were identified quickly, precisely and reliahly. In this method, infrared fingerprint spectra possess the similar ability with chromatograph fingerprint spectra in identification of traditional Chinese compound formulae. The results show that the new visual comparison method is suitable for identifying traditional Chinese compound formulae with the same dosage-form and similar composition.

关 键 词：指纹图谱 鉴定 右归丸 济生肾气丸 直观比对 电子能谱 

分 类 号：R917[医药卫生—药物分析学]