以利多卡因为探针共振光散射法测定血清白蛋白的含量  

Determination of Serum Proteins by Resonance Light Scattering Method with Lidocaine as a Probe

作　　者：王珺瑫[1] 宋双居[2] 吴秋华[2] 王春[2] 王志[2] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001 [2]河北农业大学理学院,河北保定071001

出　　处：《光谱学与光谱分析》2011年第9期2517-2520,共4页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金项目(31071580)资助

摘　　要：实验发现在十二烷基苯磺酸钠(SDBS)存在下,利多卡因能增强血清白蛋白的共振光散射强度,据此,建立了以利多卡因为探针,利用共振光散射法测定牛血清白蛋白(BSA)和人血清白蛋白(HSA)含量的新方法。考察了反应时间、试剂的加入顺序、pH值、SDBS和利多卡因的浓度以及共存干扰物等因素对共振光强度的影响。在优化的条件下,测定BSA和HSA的线性范围分别为1.0～45.0和0.5～30.0 mg.L-1。该方法用于人血清样品中蛋白含量的分析,获得了较高的精密度和准确度,五次平行测定的相对标准偏差在4.9%～5.7%之间,加标回收率在90%～103%之间。该方法使用常规荧光仪和常用化学试剂即可测定,操作简便、具有较高的灵敏度(检出限为0.14 mg.L-1)。对新鲜的人血清样品可以直接进行分析,无需进行样品前处理。本方法为人血清样品中蛋白含量的测定提供了一个可供选择的新途径。A new method for the determination of bovine serum albumin （BSA） and human serum albumin （HSA） was developed by using resonance light scattering （RLS） technique via an interaction of serum albumin with lidocaine and sodium dodecyl- benzene sulphonate （SDBS）. The RLS intensity of serum albumin was enhanced in the presence of lidocaine and SDBS. The influences of some experimental factors, including incubation time, addition sequence of reagents, pH values, foreign substances and the concentrations of lidocaine and SDBS, on the enhancement of the RLS intensity were investigated. Under the optimal conditions, the enhanced RLS intensities were proportional to the concentrations of serum albumin in the range of 1.0-45.0 mg · L^-1 for BSA and 0. 5-30. 0 mg · L^-1 for HSA. The method was successfully applied to the determination of real human ser- um samples, with the relative standard deviations of 4.9%-5.7 % （n = 5） and standard addition recoveries of 90 %-103%. The method only involves the use of conventional fluorescence spectrometer and chemical reagents. It is simple, easy to operate and sensitive with the limit of detection of 0. 14 mg · L-1. The fresh human serum samples can be directly analyzed without the need of any prior pretreatment. The method can be a good alternative of choice for the determination of BSA and lISA.

关 键 词：BSA HSA 共振光散射 利多卡因 测定 

分 类 号：O641.3[理学—物理化学]