过氧化物酶体增生激活型受体α(PPAR-α)在急性冠脉综合征(ACS)外周血淋巴细胞的表达状态及其与细胞TNF-α及hs-CRP的相关性研究  

EXPRESSION OF PPAR-Α IN ACS LYMPHOCYTES AND ITS CORRELATION WITH TNF-Α AND HS-CRP IN SERUM

作　　者：何喜民[1] 马瑞莲[1] 李天发[1] 钟江华[1] 

机构地区：[1]海南医学院心血管病研究所,海南医学院附属医院心内科,海口570102

出　　处：《现代预防医学》2011年第16期3278-3279,3281,共3页Modern Preventive Medicine

基　　金：海南省卫生厅资助课题 (琼卫 2007-010)

摘　　要：[目的]检测过氧化物酶体增生激活型受体α(PPAR-α)在外周血淋巴细胞中的表达以及观察急性冠脉综合征时PPAR-α的表达状态及其与肿瘤坏死因子(TNF-α)、超敏反应蛋白(hs-CRP)的相关性。[方法]采用ELISA法检测其47例急性冠脉综合征患者和23例正常对照组淋巴细胞中PPAR-α的表达,同时检测血清中TNF-α和hsCRP。[结果]PPAR-α在人外周血淋巴细胞膜中表达,与对照组比较,急性冠脉综合征患者外周血淋巴细胞膜PPAR-α表达显著下降(22.51±6.78vs45.44±5.20,P﹤0.01);血清TNF-α及hsCRP表达升高;PPAR-α的表达水平与TNF-α及hsCRP呈负相关(γ分别为-0.354,-0.497,P﹤0.01),而对照组中PPAR-α的表达水平与TNF-α及hsCRP无关。[结论]急性冠脉综合征时外周淋巴细胞PPAR-α表达下调,与TNF-α及hsCRP呈负相关,提示PPAR-α可能通过炎症反应参与急性冠脉综合征发病过程,并与斑块的不稳定状态有关。[Objective]To investigate the level of peroxisome proliferator-activated receptorsα（PPARα）expression in peripheral blood lymphocytes of patients with acute coronary syndrome（ACS）,and to analyze the correlation among levels of PPAR-α,matrix TNF-α and hs-CRP.[Methods]By ELISA,PPAR α- of the lymphocytes of 47 ACS patients and 23 normal cases were detected,and the TNF-α and hs-CRP of the serum were detected.[Results]The expression of PPAR α in peripheral lymphocytes in ACS was significantly lower than that in controls（22.51±6.78 vs 45.44±5.20,P﹤0.01）. PPAR α was inversely associated with TNF-α（r = -0.354,P﹤0.01）and hs-CRP（r = -0.497,P﹤0.01）.[Conclusion]The expression of PPAR-α is significantly reduced in peripheral lymphocytes in ACS,which is inversely associated with TNF-α and hs-CRP. These results suggest that PPAR α may involve in the process of ACS.

关 键 词：过氧化物酶体增生激活型受体α 外周血淋巴细胞 急性冠状动脉综合征 肿瘤坏死因子 超敏C反应蛋白 

分 类 号：R541.4[医药卫生—心血管疾病]