促卵泡激素结合片段通过PI3K/Akt-cyclinD1通路抑制卵巢癌细胞增殖活性  

Follicle-Stimulating Hormone Synthetic Peptides Inhibits Proliferation of Ovarian Cancer Cells by PI3K/Akt-cyclinD1 Pathway

作　　者：吴凌[1] 魏桠楠[1] 杨婷[1] 周冬梅[1] 祝颖[1] 冯怡雯[1] 张箴波[1] 祝亚平[1] 丰有吉[1] 

机构地区：[1]上海交通大学附属上海市第一人民医院,上海200080

出　　处：《现代生物医学进展》2011年第16期3030-3033,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金(81001155和81020108027);上海市卫生局面上项目(2009028)

摘　　要：目的:研究促卵泡激素(FSH)结合片段对卵巢上皮性细胞癌细胞株hey增殖活性的影响。方法:应用卵巢上皮性细胞癌细胞株hey作为实验对象,分别加入FSH、FSH结合片段、FSH+FSH结合片段。用四甲基偶氮唑蓝(MTT)法检测卵巢癌hey细胞的生长状况,Western blot检测cyclinD1、Akt、pAkt分子的表达。结果:FSH对卵巢癌hey细胞的生长有明显的促进作用,细胞生长活性提高21%。FSH结合片段对卵巢癌细胞的生长有明显抑制作用,对细胞平均抑制率为22%,且能下调cyclinDI的表达,在2.5×10^(-9)Mol/L达70%。FSH结合片段对FSH有竞争抑制作用,细胞抑制率为18%,能抑制FSH上调cyclinD1的作用,抑制率为61%。FSH能上调pAkt的表达,对Akt的表达没有明显影响,在40mIU/ml的浓度时pAkt/Akt上调率达224%。FSH结合片段对Akt的表达没有明显影响,但能明显下调pAkt的表达,且呈时间依赖性(在1 5分钟时达66%)和剂量依赖性(在2.5×10^(-9)Mol/L达31%)。FSH结合片段能抑制FSH上调pAkt的作用,抑制率为80%。结论:FSH结合片段可通过抑制FSH诱导的PI3K/Akt-cycl-inD1信号通路进而抑制上皮性卵巢癌hey细胞的增殖活性。Objective： To observe the inhibit effect of follicle-stimulating hormone （FSH） synthetic peptides （FSH binding Fragment） on the human epithelial ovarian cancer cell. Methods： Human epithelial ovarian cancer cell line hey was incubated with FSH, FSH binding fragrnent,FSH and FSH binding fragment respectively.The cell proliferation was detected by methyl thiazolyl tetrazolium （MTT） techniquel.cyclinD 1 ,Akt,pAkt were detected by western blot. Results： The proliferation of hey was increased apparently by FSH in a dose-dependent manner and the rate of proliferation average value 21% .Follicle-stimulating hormone （FSH） synthetic peptides inhibited the growth activity of hey,the rate of inhibition average value 22%.And the expression of cyclinD 1 was also inhibited up70% at the concentration of 2.5×10^-9 Mol/L.Follicle-stimulating hormone （FSH） synthetic peptides inhibited the effect of FSH on hey,included the growth activity （18%）and the expression of cyclinD1 （61%）.FSH did not have effect on Akt,but pAkt was increased apparently,and pAkt/Akt was elvated 224% at the concentration of 40mlU/ml.Follicle-stimulating hormone （FSH） synthetic peptides decreased the pAkt in a time-dependent manner（almost 31% at 15 minutes） and in a dose-dependent manner（almost 66% at 2.5×10^-9 Mol/L） ,but it also did not have effect on Akt. Follicle-stimulating hormone（FS;H） synthetic peptides could inhibit the effect of FSH on pAkt（80%） .Conclusions： It is suggested that FSH binding fragment can inhibit the proliferation of ovarian cancer cell stimulated by FSH though the PI3K/Akt-cyclinD 1 pathway.

关 键 词：促卵泡激素 促卵泡激素结合片段 AKT 

分 类 号：R711[医药卫生—妇产科学] Q75[医药卫生—临床医学]