赫赛汀联合姜黄素对卵巢癌细胞系SKOV3的抑制作用  

Synergistic Inhibitory Effect of Curcumin Combined with Herceptin on Human Ovarian Cancer Cell Line SKOV3

作　　者：司马玲[1] 姜群英[1] 刘彩霞[1] 冯静[1] 冯桂萍[1] 

机构地区：[1]兰州军区乌鲁木齐总医院妇产科,新疆乌鲁木齐830000

出　　处：《现代生物医学进展》2011年第16期3080-3083,共4页Progress in Modern Biomedicine

摘　　要：目的:探讨赫赛汀联合姜黄素对HER2阳性卵巢癌细胞系SKOV3增殖能力的影响。方法:体外培养至对数生长期的人卵巢癌细胞系SKOV3细胞分为对照组、赫赛汀治疗组、姜黄素治疗组以及赫赛汀联合黄素治疗组,利用四唑盐(MTT)比色法和平板集落形成试验比较各组细胞增殖能力,利用AnnexinV-FITC/PI染色比较各组细胞凋亡。结果:赫赛汀联合姜黄素治疗组SKOV3细胞活力明显低于对照组及单一药物处理组,平板集落形成试验结果表明各组细胞集落形成率分别为83.4%、36.8%、59.2%和24.7%,赫赛汀联合姜黄素处理组SKOV3细胞集落形成能力显著低于另外3组(P<0.01),Annexin V-FITC/PI染色并用流式细胞仪分析表明各组SKOV3细胞早期凋亡率分别为1.3%、26.4%、9.3%和39.1%,赫赛汀联合姜黄素处理组细胞早期凋亡率显著低于其余3组(P<0.01)。结论:赫赛汀联合姜黄素能够抑制卵巢癌细胞系SKOV3的增殖,这种抑制作用是通过促进肿瘤细胞凋亡实现的。Objective： To investigate the effects of Herceptin combined with curcumin on human ovarian cancer cell line SKOV3 in vitro. Methods： SKOV3 cells were treated with Herceptirt, curcumin, Herceptin and curcumin, respectively. Cell viability was tested by MTT assay and colony-forming assay. Results： Cell viability of SKOV3 cells treated by Herceptin combined with eureumin was lower than that of Herceptin or curcumin alone. The colony forming efficiency of each group cells was 83.4%, 36.8%, 59.2% and 24.7%, respectively （P〈：0.01）. Herceptin combined with curcumin can repress the colony-forming ability more effectively than other groups. The apoptosis rate of each group cells was 1.3%, 26.4%, 9.3% and 39.1% respectively. （P〈0.01）. Herceptin combined with curcumin can promote SKOV3 cells apoptosis. Conclusions： Herccptin combined with curcumin can inhibit the proliferation of ovarian cancer cell line SKOV3 and induce apoptosis.

关 键 词：姜黄素 赫赛汀 卵巢癌 凋亡 

分 类 号：R711.75[医药卫生—妇产科学] R737.31[医药卫生—临床医学]