DSCR1基因与喉咽癌血管新生关系的研究  

Relationship between the DSCR1 gene and angiogenesis in laryngopharynx cancer

作　　者：吕超[1] 潘新良[1] 高举[2] 李臻[2] 

机构地区：[1]山东大学齐鲁医院耳鼻咽喉科,济南250012 [2]山东烟台市烟台山医院耳鼻咽喉科,山东烟台264001

出　　处：《山东大学学报（医学版）》2011年第8期104-107,112,共5页Journal of Shandong University:Health Sciences

摘　　要：目的观察DSCR1基因在喉咽癌组织和癌旁组织中的表达及与微血管密度(MVD)之间的关系,探讨DSCR1基因对喉咽癌的血管新生的生物学行为的影响。方法收集喉咽鳞状细胞癌患者94例,其中78例取癌旁组织,通过免疫组织化学P-V9000法,使用兔抗DSCR1抗体DCT3,检测DSCR1基因在喉咽鳞状细胞癌与正常癌旁组织中的表达,通过CD34抗体染色标记血管内皮细胞,计算MVD,将DSCR1基因表达与MVD之间做相关性分析。结果肿瘤组织中DSCR1基因表达阳性率94.9%,癌旁组织表达阳性率35.9%,差异有统计学意义(t=23.69,P<0.001);喉咽癌组织平均MVD(17.57±10.61)条/400倍视野,癌旁正常组织(12.61±7.67)条/400倍视野(P<0.05)。在喉咽癌肿瘤组织中随着DSCR1基因的表达增强,MVD也逐渐升高(P<0.001),两者呈正相关性(r=0.698)。结论 DSCR1基因在喉咽鳞状细胞癌中表达增加,并与其血管生成的生物学行为密切相关,可以作为一个新的基因治疗靶向。Objective To observe expression of the down syndrome candidate region 1 （DSCR1） gene and its relationship with microvascular density （MVD） in laryngopharynx cancer and peri-cancerous tissues, and to explore the influence of the DSCR1 gene on angiogenesis in laryngopharynx cancer. Methods 94 cases of laryngopharynx squamous cell carcinoma were collected, and peri-cancerous tissues were investigated in 78 cases of them. The imrnunohistochemical P-V9000 method was used to determine expression of the DSCR1 gene with the rabbit DSCR1 antibody DCT3, and vascular endothelial cells were stained to calculate MVD values with the CD34 antibody. Relationship between expression of the DSCR1 gene and MVD was statistically analyzed. Results Positive expression rates of the DSCR1 gene were 94.9% in tumor tissues, and 35.9% in peri-cancerous tissues, and the difference was statistically significant（ t = 23.69, P 〈0.001 ）. MVD was （ 17.57 _＋ 10.61 ） bars/400 time view in laryngopharynx cancer tissues, and （ 12.61- 7.67 ） bars / 400 time view in peri-cancerous tissues （ P 〈 0.05 ）. In laryngopharynx squarnous cell carcinoma tissues, there was a positive correlation between expression of the DSCR1 gene and MVD （r =0. 698, P 〈0. 001 ）. Conclusion Expression of the DSCR1 gene in laryngopharynx squamous cell carcinoma is significantly increased, and it is closely related to angiogenesis in laryngopharynx cancer. The DSCR1 gene can be used as a new target in gene therapy.

关 键 词：DSCR1基因 喉咽鳞状细胞癌 血管新生 免疫组织化学 

分 类 号：R766.5[医药卫生—耳鼻咽喉科]