荧光定量RT-PCR检测重楼功能基因表达的差异  

Differential expression of functional genes in Paris L.as revealed by quantitative competitive RT-PCR

作　　者：刘军[1] 刘学端[1] 江雪梅[1] 廖立琴[1] 

机构地区：[1]中南大学资源加工与生物工程学院,湖南长沙410083

出　　处：《中国现代医学杂志》2011年第22期2709-2714,共6页China Journal of Modern Medicine

基　　金：国家科技基础条件平台建设项目(自然保护区生物标本标准化整理;整合及共享试点;No:2005DKA21404)

摘　　要：目的检测重楼属植物功能基因表达的差异。方法通过使用特异性引物和SYBR GreenⅠ结合染料实时荧光定量RT-PCR技术,以管家基因18 S rDNA为参照基因,采用相对定量的方法,对重楼属的3个种3部分组织的2个功能基因进行表达差异的研究。结果两个功能基因包括HMGCoA还原酶基因和鲨烯环氧酶基因在根和茎均高表达于叶,尤以根的表达量最高。结论成功将RT-PCR技术应用于药用植物的功能基因研究。通过对各种条件的优化探索,建立了准确和简单易行的检测药用植物的功能基因表达的平台。【Objective】 To detect the differences between two functional genes expression of Paris L.【Methods】 By using quantitative real-time fluorescent reverse transcriptase-polymerase chain reaction technology combined specific primers with SYBR Green Ⅰ dye stuff,referring to housekeeping gene 18 S as reference gene,the relative quantitative method was used to study the expressive differences between the two functional genes for the each part from three species of Paris L.【Results】 The experiments showed that genes HMGCoA reductase and squalene epoxidase in the root and stem were more highly expressed than in leaves with the highest expression in the root.【Conclusions】 The RT-PCR technology is applied to the functional gene research of officinal plant.Through the optimization and exploration of various conditions,an exact and easy operation platform is successfully constructed for detecting functional gene expression of officinal plant.

关 键 词：荧光定量RT-PCR 重楼 功能基因 

分 类 号：R282.5[医药卫生—中药学]