丁苯酞对脑低灌注大鼠学习记忆的改善作用  

Improvement of butylphthalide on the learning and memory in cerebral hypo-perfused rats

作　　者：罗超[1] 赵万红[1] 苗亚玲[1] 龚坚[1] 李伏燕[2] 彭吉霞[1] 

机构地区：[1]湖北医药学院基础医学院,湖北十堰442000 [2]湖北十堰太和医院,湖北十堰442000

出　　处：《中国医院药学杂志》2011年第16期1336-1339,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖北省自然科学基金资助项目(编号:2009CDZ023);湖北医药学院博士启动金(编号:2008QDJ6)

摘　　要：目的:观察丁苯酞(NBP)对脑低灌注大鼠学习记忆的改善作用,并探索其生化机制。方法:永久性结扎老龄大鼠双侧颈总动脉3个月,制备脑低灌注模型。实验分4组:假手术组、溶剂对照组、NBP30 mg.kg-1和120 mg.kg-1组。后2组连续给予NBP45 d后,用Morris水迷宫检测大鼠的学习记忆。同时,取脑组织,用生化法检测超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果:在水迷宫定位航行试验中,NBP可以显著缩短脑低灌注大鼠找到安全台的潜伏期。在空间探索试验中,NBP明显增加脑低灌注大鼠在目标象限活动时间的百分比。同时,NBP降低了大鼠脑组织代偿性增高的SOD活力和MDA水平。结论:NBP可以改善脑低灌注大鼠的学习记忆缺陷,其中减轻脑组织氧化应激损伤是重要机制。OBJECTIVE To observe the improving effects of butylphthalide（NBP） on the learning and memory of cerebral hypo-perfused rats and investigate its biochemical mechanism.METHODS Cerebral hypo-perfused models were made by ligating rats＇ bilateral common carotid arteries for three months.There were four groups：sham-operated,vehicle,NBP 30 mg·kg-1 and 120 mg·kg-1.The last two groups were administered NBP for 45 days consecutively.Rats＇ learning and memory performance were measured with Morris water maze.The superoxide dismutase（SOD） activity and the malondialdehyde（MDA） level in rats＇ brain tissue were measured with biochemical method.RESULTS In water maze positioning navigation trial,NBP reduced significantly the latencies of cerebral hypo-perfused rats＇ finding of safe platform.In spatial exploring trial,NBP increased cerebral hypo-perfused rats＇ time percentages spent in the platform-quadrant.Meanwhile,NBP reduced the compensative increasing in SOD activity and MDA level of rats.CONCLUSION NBP could improve cerebral hypo-perfused rats＇ learning and memory deficits.Ameliorating oxidative stress damage in brain tissue was its important mechanism of action.

关 键 词：丁苯酞 脑低灌注 学习记忆 超氧化物歧化酶 丙二醛 

分 类 号：R964[医药卫生—药理学]