草莓MET1基因启动子克隆及瞬时表达分析  

Isolation and Transient Expression Analysis of Strawberry MET1 Gene Promoter

作　　者：常琳琳[1,2] 张志宏[2] 张运涛[1] 李贺[2] 王桂霞[1] 张利喜[1] 

机构地区：[1]北京市农林科学院林业果树研究所,北京100093 [2]沈阳农业大学园艺学院,沈阳110866

出　　处：《西北植物学报》2011年第6期1110-1114,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(30671432)

摘　　要：根据草莓MET1类DNA甲基转移酶基因(FaMET1)全长序列设计引物,通过PCR获得了该基因翻译起始位点上游669 bp的序列。生物信息学分析表明,该序列中存在启动子的基本元件TATA-box、CAAT-box和多个胁迫诱导元件。进一步构建了FaMET1基因启动子驱动GUS的植物表达载体FMpro∷GUS,通过农杆菌注射法转化草莓果实细胞,结果表明,该序列能够驱动GUS在草莓果实中瞬时表达,为进一步研究FaMET1的表达调控奠定了基础。A 669 bp-length regulative sequence upstream 5′ of translation start site of strawberry MET1 gene（FaMET1） was cloned by PCR using the specific primers which were designed according to the full gene sequence.In silico analysis of cloned sequence revealed that it contained basic cis-elements,such as TATA-box and CAAT-box,and many stress responsive elements.Moreover,FMpro∷GUS was constructed and transformed into strawberry fruits by infection with an Agrobacterium strain.The results showed that the sequence could drive the transient expression of GUS in strawberry fruits.It could make substantial basis for further research on the mechanism of regulation and expression of FaMET1.

关 键 词：草莓 MET1 启动子 瞬时表达 

分 类 号：Q785[生物学—分子生物学]