S-腺苷甲硫氨酸代谢途径相关基因在小麦水分胁迫中的表达  

Expression of Genes Related to the S-adenosyl-L-methionine Metabolic Pathways during Water Stress in Wheat

作　　者：李昌澎[1] 周琳璘[2] 陈亮[2] 黄林周[2] 陈晓杰[2] 王宇珅 胡银岗[1,2,3,4] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨陵712100 [2]西北农林科技大学农学院,陕西杨陵712100 [3]国家小麦改良中心杨凌分中心,陕西杨陵712100 [4]陕西省农业分子生物学重点实验室,陕西杨陵712100

出　　处：《西北植物学报》2011年第6期1120-1126,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：‘863’重点项目子课题(2006AA100201;2006AA100223);科技部973计划前期项目(2006CB708208);高等学校学科创新引智计划项目(111-2-16);澳大利亚ACIAR项目(CIM/2005/111)

摘　　要：以小麦品种‘晋麦47’为材料,利用半定量RT-PCR方法,对S-腺苷甲硫氨酸代谢途径中的S-腺苷甲硫氨酸合成酶（SAMS）基因、S-腺苷甲硫氨酸脱羧酶（SAMDC）基因和γ-谷氨酰半胱氨酸合成酶（-γECS）基因在正常供水、PEG-6000模拟水分胁迫和复水过程中小麦叶片的表达模式进行了分析。结果表明,3个基因在正常生长情况下有一定量的表达,SAMS和SAMDC基因在水分胁迫早期（PEG-6000胁迫6、12、244、8 h）上调表达,水分胁迫后期（PEG-6000胁迫75 h）表达量下降;复水后3~6 h上调表达,复水9 h后表达量下调至对照水平。-γECS基因在水分胁迫阶段呈上调表达,复水后表达量下调至对照水平。可见,小麦SAMS、SAMDC和-γECS基因的表达都受水分胁迫诱导,同时,SAMS与SAMDC基因还参与水分胁迫后的复水调节,说明S-腺苷甲硫氨酸代谢途径在小麦抗旱节水中具有重要作用。To study the roles of the S-adenosyl-L-methionine metabolic pathways in drought-tolerance and water use efficiency of wheat,we deployed the semi-quantitative RT-PCR to investigate the expression patterns of the three genes of S-adenosylmethionine synthetase,S-adenosylmethionine decarboxylase and gamma-glutamylcysteine synthetase in the leaves of wheat seedling of Jinmai 47 during PEG-simulated water stress and re-watering after serious water stress.The results showed that the three genes were expressed under the normal growth condition.The expressions of SAMS and SAMDC were up-regulated at 6,12,24 and 48 h of PEG-6000 simulated water stress,then were down-regulated at 75 h of water stress,and were up-regulated again at 3～6 h after re-watering,decreased again after 9 h after re-watering to the normal level.The expressions of γ-ECS were up-regulated at PEG-6000 simulated water stress,then were down-regulated at re-watering to the normal level.The expression patterns of wheat SAMS,SAMDC and γ-ECS during water stress and re-watering after serious water stress indicated that the three genes were involved in the regulations of wheat to water stress,and SAMS and SAMDC were also involved in the recovery after re-watering.The results suggested that the S-adenosyl-L-methionine metabolic pathways played an important role in drought-tolerance and water use efficiency in wheat.

关 键 词：小麦 水分胁迫 S-腺苷甲硫氨酸代谢途径 表达模式 S-腺苷甲硫氨酸合成酶(SAMS) S-腺苷甲硫氨酸脱羧酶(SAMDC) γ-谷氨酰半胱氨酸合成酶(-γECS) 

分 类 号：Q789[生物学—分子生物学]