Cu^(2+)胁迫对2种速生柳幼苗生长及生理特性的影响  

Growth and Physiological Characteristics of Salix jiangsuensis and Salix babylonica Seedling under Cu^(2+) Stress

作　　者：刘治昆[1,2] 陈彩虹[2] 陈光才[1] 张建锋[1] 

机构地区：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400 [2]中南林业科技大学林学院,长沙410004

出　　处：《西北植物学报》2011年第6期1195-1202,共8页Acta Botanica Boreali-Occidentalia Sinica

基　　金：中央级公益性科研院所基本科研业务费专项资金重点项目(RISF6803);江苏省科技支撑计划项目(BE2008636;BE2009603)

摘　　要：以营养溶液中培养的‘苏柳172’(Salix jiangsuensis CL J-172)和垂柳(Salix babylonica Linn)幼苗为材料,分析它们在不同浓度Cu2+溶液(0、20、40、80、100、200、300μmol.L-1)下的生长以及部分生理指标的变化,以明确2种速生柳树用于植物修复的潜力。结果显示:2种柳树幼苗的生物量、叶片色素含量、根系活力以及根系形态的各指标都随Cu2+浓度的增加而显著降低。它们叶片SOD和POD活性以及脯氨酸含量随Cu2+浓度的增加呈先上升后下降的趋势,‘苏柳172’和垂柳中SOD活性在Cu2+浓度为20μmol.L-1时最大,其POD活性分别在Cu2+浓度为100和40μmol.L-1时达到最大,而其脯氨酸含量在Cu2+浓度为200μmol.L-1时达到最大。可见,2种柳树生长均受到Cu2+胁迫的抑制,它们能够在一定程度上通过增加保护酶活性和脯氨酸含量来提高对Cu2+的耐受性,且‘苏柳172’对Cu2+的忍耐力强于垂柳。In order to evaluate the potential of phytoremediation application of fast-growing willows,we investigated the growth and the changes of some physiological indexes for seedlings of Salix jiangsuensis CL J-172 and Salix babylonica grown in hydroponic solution under different copper concentrations.The results showed that the biomass,chlorophyll contents in leaves,root activities and root morphology indexes decreased obviously as copper concentration increased.The activities of SOD,POD and proline contents first increased and then decreased as the copper concentration increased.The activity of SOD in S.jiangsuensis CL J-172 and S.babylonica reached the highest value when the copper concentration was 20 μmol·L-1,the activity of POD reached the highest value when the copper concentration were 100 μmol·L-1 for S.jiangsuensis CL J-172 and 40 μmol·L-1 for S.babylonica and the proline content reached the maximum when the copper concentration was 200 μmol·L-1.It could be concluded that the growth of two species of willows were both inhibited by copper stress,they can increased the SOD activity,POD activity and proline content to resist this stress,and S.jiangsuensis CL J-172 had better endurance than S.babylonica.

关 键 词：CU2+ 速生柳 保护酶 光合色素 根系形态 

分 类 号：Q945.78[生物学—植物学]