棉花大田植株叶柄组织培养体系的建立  

Plantlets Regeneration from Mature Leaf's Petiole of Cotton

作　　者：张朝军[1] 范术丽[1] 武芝霞[1] 张雪妍[1] 王玉芬[1] 李付广[1] 

机构地区：[1]中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,河南安阳455000

出　　处：《西北植物学报》2011年第6期1257-1263,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：“863”优质高产棉花分子品种创制(2006AA100105)

摘　　要：以早熟棉花品种‘中棉所24’为材料,通过对大田棉花植株叶柄灭菌条件、叶柄愈伤组织诱导与分化、胚状体的萌发及植株再生所需的培养基等进行研究,以建立棉花大田植株叶柄组织培养体系。结果表明：棉花植株叶柄经0.1%HgCl2灭菌4~5 min后,横切成0.8 cm左右的小段,在MSB＋0.05 mg·L-1IAA＋0.05 mg·L-1KT＋0.05 mg·L-12,4-D＋25 g·L-1葡萄糖＋2 g·L-1Gel（pH 5.8）培养基上培养,易获得分化的愈伤组织;愈伤组织在添加有KT/IAA（MSB＋0.08 mg·L-1IAA＋0.08 mg·L-1KT＋25 g·L-1葡萄糖＋2 g·L-1Gel,pH6.5）或ZT/IBA（MSB＋0.1 mg·L-1ZT＋0.1 mg·L-1IBA＋25 g·L-1葡萄糖＋2 g·L-1Gel,pH 6.5）的分化培养基中,均可以获得胚性愈伤组织。胚性愈伤组织在MSB＋0.05 mg·L-16-BA＋0.05 mg·L-1IAA＋25 g·L-1蔗糖＋2 g·L-1Gel（pH 6.5）培养基上,形成胚状体、生长发育成再生植株。The study was to stablish a tissue culture system with petioles of grown-in-field cotton plants as explants,using cotton variety CCRI 24 as materials by the analysis the sterilization conditions for petioles of grown-in-field cotton plants,the induction and differentiation of petiole callus,the culture medium used for embryoid germination and the plant regeneration.The results showed that sterilization requirement can be reached with petioles soaked in 0.1% HgCl2 for 4 to 5 minutes.Easily differentiated callus can be obtained with 0.8 cm sterilized segments cultured in the medium containing MSB＋0.05 mg·L-1 IAA＋0.05 mg·L-1 KT＋0.05 mg·L-1 2,4-D＋25 g·L-1 glucose＋2 g·L-1 Gel（pH 5.8）.Petiole callus are able to differentiate and change into embryogenic callus in two kinds of culture media,respectively,containing either KT/IAA or ZT/IBA.The favorable medium for the differentiation are MSB＋ 0.08 mg·L-1 IAA＋0.08 mg·L-1 KT＋25 g·L-1 glucose＋2 g·L-1 Gel（pH 6.5） and MSB＋0.1 mg·L-1 ZT＋0.1 mg·L-1 IBA＋25 g·L-1 glucose＋2 g·L-1 Gel（pH 6.5）.Embryogenesis callus can develop into embryoid and then into plantlets in the medium containing MSB＋0.10 mg·L-1 6-BA＋0.05 mg·L-1 IAA＋25 g·L-1 sucrose＋2 g·L-1 Gel（pH 6.5）.

关 键 词：棉花 叶柄 组织培养 

分 类 号：Q813.1[生物学—生物工程]